基于PTCDA/TiO_(2)敏化结构构建灵敏光电化学传感器  

A sensitive photoelectrochemical sensor based on PTCDA/TiO_(2) sensitized structure

作　　者：周游 陈杨 沈国豪 李孟洁 Zhou You;Chen Yang;Shen Guo-hao;Li Meng-jie(School of Civil Engineering and Architecture Chongqing University of Science&Technology,Chongqing 401331,China)

机构地区：[1]重庆科技学院建筑工程学院,重庆401331

出　　处：《化学传感器》2023年第4期54-61,共8页Chemical Sensors

基　　金：重庆科技学院硕士研究生创新计划项目(YKJCX2220601)。

摘　　要：该文基于3,4,9,10-苝四羧酸二酐(PTCDA)对二氧化钛(TiO_(2))的高效敏化效应,设计了一种用于灵敏检测C反应蛋白(CRP)的光电化学(PEC)适体传感器。首先,TiO_(2)和PTCDA在电极表面上连续孵育形成PTCDA/TiO_(2)敏化结构,以产生强烈的PEC信号。随后,在已孵育完成的电极上电沉积金纳米粒子(dep Au),通过Au-N键与CRP适体(S1)连接。然后,在电极表面添加1-己硫醇(HT)以阻断非特异性吸附位点。最终,加入的CRP和S1特异性识别产生CRP-S1复合物,使PEC信号明显降低。在最佳条件下,所提出的PEC传感器实现了对CRP的灵敏检测,其线性范围为10 pg/mL-10μg/mL,检测限低至3.33 pg/mL。Herein,a photoelectrochemical(PEC)aptasensor for sensitive detection of C-reactive protein(CRP)was designed based on the efficient sensitization effect of 3,4,9,10-perylenetetracarboxylic dianhydride(PTCDA)to titanium dioxide(TiO_(2)).Initially,TiO_(2) and PTCDA were successively incubated on the electrode surface to form a PTCDA/TiO_(2) sensitized structure,which generated a strong PEC signal.Subsequently,gold nanoparticles were electrodeposited(dep Au)on electrode surface,which could connect to CRP aptamer(S1)via Au-N bonds.Then,1-hexanethiol(HT)was added to the surface of the modified electrode to block the non-specific adsorption sites.Ultimately,with the addition of CRP,the CRP-S1 complex was produced by the specific recognition of CRP and S1,resulting in a significant reduction of PEC signal.Under the optimal conditions,the proposed PEC sensor was successfully constructed for sensitive detection of CRP with a wide linear range of 10 pg/mL-10μg/mL and a low detection limit 3.33 pg/mL.

关 键 词：光电化学适体传感器 二氧化钛 3 4 9 10-苝四羧酸二酐 敏化效应 C反应蛋白 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置] O657.1[自动化与计算机技术—控制科学与工程]