基于NIRS快速检测高糖高脂饮食的果蝇体内抗氧化酶活性  

Rapid Determination of Antioxidant Enzyme Activity in Drosophila Melanogaster Induced by High-Sugar or High-Fat Diets Based on NIRS

作　　者：黄佳敏 佟海滨 吴郁 刘剑 杨越 HUANG Jiamin;TONG Haibin;WU Yu;LIU Jian;YANG Yue(College of Life and Environmental Science,Wenzhou University,Wenzhou,China 325035;Key Laboratory of Water Environment and Marine Biological Resources Protection of Zhejiang Province,Wenzhou,China 325035)

机构地区：[1]温州大学生命与环境科学学院,浙江温州325035 [2]浙江省水环境与海洋生物资源保护重点实验室,浙江温州325035

出　　处：《温州大学学报（自然科学版）》2024年第2期27-36,共10页Journal of Wenzhou University(Natural Science Edition)

基　　金：国家自然科学基金项目(31900274);浙江省自然科学基金项目(LTGN23C020001)。

摘　　要：基于近红外光谱技术(NIRS)构建一种快速检测不同饮食条件下果蝇体内抗氧化酶活性的分析方法.收集240个高糖、高脂饮食条件下果蝇幼虫样品,分别通过硫代巴比安酸比色法和羟胺法测定过氧化氢酶(CAT)及超氧化物歧化酶(SOD)活性,采集果蝇提取液近红外光谱(NIR);基于NIR和两种抗氧化酶活性测定值构建偏最小二乘(PLS)定量校正模型,并比较不同的光谱预处理方法和波段对模型性能的影响.结果显示,高糖组的CAT和SOD的PLS定量校正模型的预测集相关系数(RP)及预测集误差均方根(RMSEP)分别为0.982、2.34 U/gprot和0.983、0.86 U/gprot;高脂组的CAT和SOD的PLS定量校正模型的参数RP及RMSEP分别为0.976、3.01 U/gprot和0.969、2.16 U/gprot,预测结果良好.证实利用NIRS可以快速检测果蝇体内的抗氧化酶活性.To develop a rapid NIRS methodology for the measurement of antioxidant enzyme activity in Drosophila melanogaster under different diet conditions,240 Drosophila samples were collected under high-sugar or high-fat diets and the activity of two antioxidant enzymes,catalase(CAT)and superoxide dismutase(SOD),was determined by the thiobarbituric acid colorimetric method and hydroxylamine method,respectively.The near-infrared spectrum(NIR)of Drosophila extract was collected.A partial least squares(PLS)quantitative correction model was constructed based on NIR and two antioxidant enzyme activity reference values,and the effects of different spectral pretreatment methods and bands on the model performance were compared.The overall results had satisfactory prediction performance.For Drosophila induced by high-sugar diets,the correlation coefficient(RP)and root mean square error of prediction(RMSEP)of the PLS quantitative correction model were 0.982 and 2.34 U/gprot for CAT and 0.983 and 0.86 U/gprot for SOD,respectively;for Drosophila induced by high-fat diets,the RP and RMSEP of the PLS quantitative correction model were 0.976 and 3.01 U/gprot for CAT and 0.969 and 2.16 U/gprot for SOD,respectively.It was proved that NIRS could be successfully applied to rapidly detect the activity of antioxidant enzymes in Drosophila melanogaster.

关 键 词：近红外光谱技术 果蝇 抗氧化酶活性 快速分析 

分 类 号：O657.33[理学—分析化学]