一例赤大袋鼠梭菌性肠炎的诊断和病原分离鉴定  

Diagnosis and Pathogen Isolation of a Case of Clostridial Enteritis in Red Kangaroo

作　　者：董俊斌[1] 李静 丁玉林[1] 王文龙[1] 高娃 郑秉武 张迪 王金玲[1] DONG Junbin;LI Jing;DING Yulin;WANG Wenlong;GAO Wa;ZHENG Bingwu;ZHANG Di;WANG Jinling(College of Veterinary Medicine,Inner Mongolia Agricultural University,Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease,Ministry of Agriculture and Rural Affairs,Hohhot,010010,China;Inner Mongolia Science and Technology Agency,Hohhot,010000,China;Inner Mongolia Key Laboratory of Tick-Borne Zoonotic Infectious Disease,Department of Medicine,College of Hetao,Bayan Nur,015000,China;Hohhot Zoo,Hohhot,010050,China;Workstation for Animal Husbandry and Aquaculture in Horqin District,Tongliao,028000,China)

机构地区：[1]内蒙古农业大学兽医学院,农业农村部动物疾病临床诊疗技术重点实验室,呼和浩特010010 [2]内蒙古自治区科学技术厅,呼和浩特010000 [3]内蒙古自治区蜱媒人畜共患传染病重点实验室,河套学院医学系,巴彦淖尔015000 [4]呼和浩特市动物园,呼和浩特010050 [5]内蒙古通辽市科尔沁区畜牧水产工作站,通辽028000

出　　处：《野生动物学报》2024年第2期403-408,共6页CHINESE JOURNAL OF WILDLIFE

基　　金：内蒙古自治区科技重大专项课题(2021ZD0012)。

摘　　要：通过临床症状、尸体剖检、组织病理学、毒素实验和细菌分离鉴定的方法确诊了1例由A型产气荚膜梭菌(Clostridium perfringens)引起的赤大袋鼠(Macropus rufus)梭菌性肠炎的自然病例。动物园1只成年赤大袋鼠突然精神沉郁、厌食,于20 h后死亡,尸体角弓反张,腹围膨大。尸体剖检和组织学检查表明:胃肠道、肝脏和肾脏出现具有梭菌性肠炎证病意义的病变。小白鼠毒素实验结果显示,血样肠内容物内有急性致死性毒素。从出血的肠黏膜面分离到单一、有芽孢、粗大的革兰阳性杆菌,经产气荚膜梭菌毒素基因型多重PCR鉴定为A型产气荚膜梭菌。研究结果可为赤大袋鼠梭菌性肠炎的诊断和防控提供依据。A natural case of clostridial enteritis in a red kangaroo(Macropus rufus)caused by Clostridium perfringens type A was diagnosed by clinical symptoms,necropsy,histopathology,toxin testing and bacterial isolation and identification.An adult red kangaroo was suddenly depressed and anorexic,and died 20 hours later,with opisthotonos and the expanded abdominal circumference in zoo.Necropsy and histologic examination revealed lesion in gastrointestinal tract,liver,and kidney that were suggestive of clostridial enteritis.Results of the mouse toxin test in revealed acute lethal toxin in the hemorrhagic intestinal contents.A single,bacillus-producing,bulky gram-positive bacillus was isolated from the mucosal surface of the hemorrhagic intestine and identified as Clostridium perfringens type A by multiplex PCR of the toxin genotype of C.perfringens.This study can provide a basis for the diagnosis,prevention and control of C.perfringens enteritis in red kangaroos.

关 键 词：赤大袋鼠 梭菌性肠炎 出血性坏死性胃肠炎 A型产气荚膜梭菌 

分 类 号：S858.9[农业科学—临床兽医学]