Pseudomonas aeruginosa infections and improper storage conditions influence the performance of 1,3‐β‐D‐glucan in diagnosis of invasive fungal infections  

作　　者：Zilan Wei Jie Xu Fang Yuan Wendong Fang Jiahui Wu Youliang Wang Shuiping Chen 

机构地区：[1]Medical School of Chinese PLA,Beijing,China [2]Department of Laboratory Medicine,5th Medical Center of Chinese PLA General Hospital,Beijing,China [3]Department of Laboratory Medicine,The PLA 307 Clinical College,5th Clinical Medical College of Anhui Medical University,Beijing,China [4]Beijing Institute of Biotechnology,Beijing,China

出　　处：《iLABMED》2024年第1期53-59,共7页智能检验医学（英文）

摘　　要：Background:The association between 1,3-β-D-glucan(BDG)levels and in-fections caused by Pseudomonas aeruginosa or Streptococcus pneumoniae,and the stability of BDG under different storage conditions are unclear.Methods:Strains of Pseudomonas aeruginosa and S.pneumoniae were grown in medium and human serum.The BDG concentrations in culture superna-tants were measured.The specificity and stability of BDG were also evaluated.Results:P.aeruginosa produced high levels of BDG in Luria-Bertani medium(>4×10^(4)pg/mL)and human serum(527.0 pg/mL),whereas S.pneumoniae produced low levels of BDG in THY medium(175.6 pg/mL)and human serum(78.3 pg/mL).The BDG produced by these two bacteria was specifically degraded by 1,3-β-D-glucanase.BDG was degraded when stored at different temperatures,decreasing by 22.5%and 9.3%at−20℃and−70℃,respectively,for 63 days;by 30.7%at 4℃for 12 days;and by 12.6%and 22.0%at 37℃for 6 and 12 h.Conclusion:BDG false-positivity must be considered in patients with bacteremia caused by P.aeruginosa when diagnosing invasive fungal infection.Human serum samples for the BDG test in medical facilities should be tested as soon as possible or stored at low temperatures before testing.

关 键 词：1 3‐β‐D‐glucan BACTEREMIA false‐positivity Pseudomonas aeruginosa STABILITY Streptococcus pneumoniae 

分 类 号：R519[医药卫生—内科学]