群体感应分子3OC12HSL对小鼠生长、血液和肠道的影响  

Effects of Quorum Sensing Molecule N-3-Oxododecanoyl-L-Homoserine Lactone(3OC12HSL)on Growth,Blood and Gut of Mice

作　　者：陈帅 高前磊 李川皓 张相伦[1] 王迎雪[3] 袁震 盛清凯[1] Chen Shuai;Gao Qianlei;Li Chuanhao;Zhang Xianglun;Wang Yingxue;Yuan Zhen;Sheng Qingkai(Institute of Animal Husbandry and Veterinary Medicine,Shandong Academy of Agricultural Sciences/Shandong Key Laboratory for Livestock and Poultry Disease Control and Breeding,Jinan 250100,China;School of Animal Science and Animal Medicine,Tianjin Agricultural University/Tianjin Key Laboratory of Agricultural Animal Reproduction and Healthy Breeding,Tianjin 300384,China;The Second Hospital of Shandong University,Jinan 250033,China;Shandong Xinji Animal Husbandry Co.,Ltd.,Taian 271200,China)

机构地区：[1]山东省农业科学院畜牧兽医研究所/山东省畜禽疫病防治与繁育重点试验室,山东济南250100 [2]天津农学院动物科学与动物医学学院/天津市农业动物繁殖与健康养殖重点试验室,天津300384 [3]山东大学第二医院,山东济南250033 [4]山东鑫基牧业有限公司,山东泰安271200

出　　处：《山东农业科学》2024年第4期159-163,共5页Shandong Agricultural Sciences

基　　金：山东省重点研发计划项目(2022TZXD0016);国家自然科学基金项目(31772649)。

摘　　要：本研究旨在探讨群体感应分子3OC12HSL对小鼠生长、炎症因子及肠粘膜屏障的影响。采用高剂量盐酸林可霉素灌胃方法建立小鼠腹泻模型,对正常小鼠和腹泻模型小鼠分别灌胃生理盐水、二甲基亚砜(DMSO)、溶于DMSO的3OC12HSL溶液。结果显示,灌胃3OC12HSL降低正常小鼠和腹泻小鼠的增重,提高血清中D-乳酸、TNF-α含量,降低大肠长度及空肠和回肠绒毛长度(P<0.05);腹泻提高血清中IL-1、IL-8、TNF-α含量,降低空肠及回肠绒毛长度(P<0.05);灌胃3OC12HSL和腹泻对回肠绒隐比有协同降低趋势,但差异不显著(P>0.05)。综上,3OC12HSL不利于小鼠的生长,腹泻可能加重3OC12HSL的影响。This study aimed to investigate the effects of quorum sensing molecule N-3-oxododecanoyl-L-homoserine lactone(3OC12HSL)on growth,inflammatory factors and intestinal mucosal barrier of mice.The mice diarrhea model was established by intragastric administration of high dose lincomycin hydrochloride solution.Normal and diarrhea mice were administrated intragastrically with normal saline,dimethyl sulfoxide(DMSO)or 3OC12HSL dissolved in DMSO,respectively.The results showed that intragastric administration of 3OC12HSL decreased weight gain of normal and diarrhea mice,increased serum D-lactate and TNF-α con-centrations,and decreased the length of large intestine and the villus length of jejunum and ileum(P<0.05).Diarrhea increased IL-1,IL-8 and TNF-α concentrations in serum,decreased the villus length of jejunum and ileum(P<0.05).3OC12HSL and diarrhea showed synergistically decreasing trend on the ratio of ileal villus length to crypt depth(P>0.05).In summary,3OC12HSL was not benefit to mice growth,and the influence might be exacerbated by diarrhea.

关 键 词：3OC12HSL 小鼠 腹泻 生长 血液 肠道 粘膜屏障 

分 类 号：S856.61[农业科学—临床兽医学]