荧光检测器高效液相色谱法测定樟脑丸中萘  

Determination of naphthalene in mothballs by high performance liquid chromatography with fluorescence detector

作　　者：张茹月 谢超[1] 张璐璐 于文斌 郭新颖[1] ZHANG Ruyue;XIE Chao;ZHANG Lulu;YU Wenbin;GUO Xinying(Nantong Center for Disease Control and Prevention,Nantong 226001,China)

机构地区：[1]南通市疾病预防控制中心,江苏南通226001

出　　处：《化学分析计量》2024年第4期13-17,共5页Chemical Analysis And Meterage

基　　金：江苏省中医药局项目(YB2020068);南通市卫健委青年基金项目(QA2021054);南通市科技计划基础科学研究项目(JC 2021076)。

摘　　要：建立高效液相色谱法测定樟脑丸中萘的含量。样品以甲醇为溶剂超声溶解,经0.22μm滤膜过滤后,以荧光检测器高效液相色谱法测定。荧光检测器激发、发射波长分别为290、330 nm,以甲醇为流动相,流量为1.0 mL/min,以Platisil ODS柱(250 mm×4.6 mm,5μm)色谱柱作为分析柱,柱温为25℃,进样体积为10μL,利用色谱峰面积外标法进行定量。该方法线性相关系数为0.9999,检出限为1.0×10^(-8)g/mL,样品加标回收率为98.4%~102.8%(n=6),测定结果的相对标准偏差为1.81%(n=6)。该方法可用于樟脑丸中萘的检测。A high performance liquid chromatography method was established for the determination of naphthalene in mothballs.The samples were dissolved ultrasonically in methanol and filtered by 0.22μm filter membrane.Then the samples were determined by high performance liquid chromatography with fluorescence detector.The excitation and emission wavelengths of the fluorescence detector were 290 nm and 330nm,respectively.Methanol was used as the mobile phase with a flow rate of 1.0 mL/min.Platisil ODS column(250 mm×4.6 mm,5μm)was used as the analytical column with the temperature of 25℃,and the injection volume was 10μL.The chromatographic peak area external standard method was used for quantitative analysis.The linear correlation coefficient of this method was 0.9999,and the detection limit was 1.0×10^(-8)g/mL.The recovery rate for spiked ranged from 98.4%to 102.8%(n=6)with the relative standard deviation of 1.81%(n=6).This method is suitable for the determination of naphthalene in mothballs.

关 键 词：萘 天然樟脑丸 高效液相色谱法 荧光检测器 

分 类 号：O657.7[理学—分析化学]