红毛五加叶水提取物对大鼠局灶性脑缺血损伤的预防作用  

Preventive Effect of Aqueous Extract of Hongmao Wujia Leaves on Focal Cerebral Ischemic Injury in Rats

作　　者：王欣[1] 吴银瓶[1] 鞠洋[1] 孟璇[1] WANG Xin;WU Yinping;JU Yang;MENG Xuan(Lanzhou University Second Hospital,Lanzhou 730030,China)

机构地区：[1]兰州大学第二医院,甘肃兰州730030

出　　处：《西部中医药》2024年第5期44-47,共4页Western Journal of Traditional Chinese Medicine

基　　金：甘肃省科技计划项目(20JR5RA326)。

摘　　要：目的:研究红毛五加叶水提取物(acanthopanax giraldii leaves extract,AGLE)对大鼠局灶性脑缺血损伤的预防作用。方法 :将138只SPF级雄性SD大鼠随机分为假手术组、模型组、银杏叶片组(0.04 g/kg)及AGLE高、中、低剂量组(20、10、5 g/kg),每组23只,采用插线法阻塞大鼠大脑中动脉(middle cerebral artery,MCA)制备局灶性脑缺血模型;采用氯化三苯基四氮唑(2.3.5-Triphenytetrya-zolium chloride,TTC)染色法测定脑梗死范围;Longa法观测大鼠神经功能缺失评分;光镜下观察缺血侧大脑皮层病理学改变;分光光度法和硝酸还原酶法分别测定脑组织一氧化氮合酶(nitric oxide synthase,NOS)活性及一氧化氮(nitric oxide,NO)含量。结果:模型组大鼠行为指标评分、脑梗死范围、NOS活性及NO含量均高于假手术组(P<0.01);缺血侧大脑皮层出现神经元结构不清,神经细胞肿胀,核固缩、溶解、破裂或消失等病理改变。与模型组比较,AGLE各剂量组大鼠行为指标评分、脑梗死范围、NOS活性及NO含量均降低(P<0.01)。与银杏叶片组比较,AGLE低剂量组改善大鼠行为指标评分更明显(P<0.01)。AGLE各剂量组脑梗死范围、NOS活性及NO含量与银杏叶片组比较,差异无统计学意义(P>0.05)。结论:AGLE对大鼠局灶性脑缺血损伤具有预防作用,其作用机制可能与降低脑组织中NOS活性及NO含量有关。Objective:To study the protective effect of aqueous extract of Hongmao Wujia leaves(acanthopanax giraldii leaves extract,AGLE)on focal cerebral ischemic injury in rats.Methods:All 138 male SD rats were randomly divided into sham operation group,the model group,ginkgo biloba group(0.04 g/kg),and high,medium,and low dose groups(20,10,and 5 g/kg)of AGLE,23 ones in each group and focal cerebral ischemia model was prepared using the middle cerebral artery occlusion(MCAO)of rats by the wire-insertion method;the extent of cerebral infarction was determined by TTC staining;the neurological deficit scores of the rats were observed by the Longa method;and the pathological changes in the cerebral cortex on the ischemic side were observed under light microscope;the nitric oxide synthase(NOS)activity and nitric oxide(NO)content of brain tissue were measured by spectrophotometry and nitrate reductase method,respectively.Results:Behavioral index scores,extent of cerebral infarction,NOS activity and NO content were higher in the model group than in the sham-operated group(P<0.01);the cerebral cortex on the ischemic side showed pathological changes such as neuronal structural ambiguity,neuronal cell swelling,and nuclear consolidation,lysis,rupture or disappearance.Behavioral index scores,extent of cerebral infarction,NOS activity and NO content were all reduced in each dose group of AGLE compared with the model group(P<0.01).Compared with ginkgo biloba group,behavioral index scores were improved more obviously in low dose group of AGLE(P<0.01).The difference had no statistical meaning in the comparisons of extent of cerebral infarction,NOS activity and NO content between different doses groups of AGLE(P>0.05).Conclusion:AGLE has a preventive effect on focal cerebral ischemic injury in rats,and its mechanism of action may be related to the reduction of NOS activity and NO content in brain tissue.

关 键 词：局灶性脑缺血 一氧化氮合酶 一氧化氮 红毛五加叶水提取物 大鼠 

分 类 号：R235.5[医药卫生—中医学]