一株肉桂炭疽病病原菌分离鉴定及生防菌筛选  

Isolation and Identification of a Pathogen of Cinnamon Anthracnose and Screening of Biocontrol Bacteria

作　　者：王洁 梁丽婷 谢欣怡 黄荣韶 姚绍嫦 谭小明 WANG Jie;LIANG Liting;XIE Xinyi;HUANG Rongshao;YAO Shaochang;TAN Xiaoming(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning,Guangxi 530200;Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine,Guangxi University of Chinese Medicine,Nanning,Guangxi 530200;Key Laboratory of Protection and Utilization of Chinese Medicine Resources,Guangxi University of Chinese Medicine,Nanning,Guangxi 530200)

机构地区：[1]广西中医药大学药学院,广西南宁530200 [2]广西中医药大学壮瑶药重点实验室,广西南宁530200 [3]广西中医药大学广西高校中药民族药资源保护与利用重点实验室,广西南宁530200

出　　处：《北方园艺》2024年第8期102-108,共7页Northern Horticulture

基　　金：广西科技重大专项资助项目(桂科AA22096029);中央引导地方科技发展资金资助项目(桂科ZY22096003);广西壮瑶药重点实验室资助项目(桂科基字[2014]32号)。

摘　　要：以广西防城港人工栽培的肉桂病叶为试材,采用内生真菌分离纯化法、形态学观察、ITS测序和平板对峙等方法,研究了肉桂炭疽病病原菌的分类地位及其拮抗菌株的筛选,以期为肉桂炭疽病生防菌开发提供参考依据。结果表明:从广西防城港肉桂病叶中分离出6株真菌(B1~B6),B6明显致病。通过形态和分子生物学鉴定该菌株为炭疽菌Colletotrichum gloeosporioides。平板对峙结果表明,RG256拮抗效果最佳,抑制率为77.05%±5.06%,鉴定为Gliocladiopsis sp.。综上所述,明确广西防城港肉桂炭疽病的病原真菌为炭疽病菌(Colletotrichum gloeosporioides),并筛选到对肉桂炭疽病病原真菌有拮抗作用的生防菌株,为肉桂炭疽病的绿色防治提供参考依据。Guangxi Fangchenggang diseased cinnamon leaves in cultivation were used as experimental materials.The taxonomic status of cinnamon anthracnose pathogen and the screening of its antagonistic strains were investigated by using the endophytic fungi isolation and purification technology method,morphological observation,ITS sequencing and plate confrontation,in order to provide reference for the development of biocontrol fungi against cinnamon anthracnose.The results showed that 6 strains(B1-B6)of fungi were isolated from cinnamon diseased leaves in Fangchenggang,Guangxi,and B6 was clearly pathogenic.The strain was identified as Colletotrichum gloeosporioides by morphology and molecular biology.The results of the tablet confrontation showed that RG256 had the best antagonistic effect,with an inhibition rate of 77.05%±5.06%,and was identified as Gliocladiopsis sp..In summary,it had been clarified that the pathogenic fungus of cinnamon anthracnose in Fangchenggang,Guangxi was Colletotrichum gloeosporioides,and biocontrol strains with antagonistic effects against the pathogenic fungi of cinnamon anthracnose had been screened,providing reference for the green control of cinnamon anthracnose.

关 键 词：肉桂 炭疽病菌 内生真菌 拮抗菌株 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]