红芝LYL263混合培养高产漆酶菌株的筛选  

Screening of High-yield Laccase Strains in Mixed Culture of LYL263 From Ganoderma lucidum

作　　者：谌斌 易小畅[1,2] CHEN Bin;YI Xiaochang(Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection,Ministry of Education,Guilin,Guangxi 541004;College of Environment and Resources,Guangxi Normal University,Guilin,Guangxi 541004)

机构地区：[1]珍稀濒危动植物生态与环境保护教育部重点实验室,广西桂林541004 [2]广西师范大学环境与资源学院,广西桂林541004

出　　处：《北方园艺》2024年第8期114-118,共5页Northern Horticulture

基　　金：国家自然科学基金资助项目(41661077);广西自然科学基金面上资助项目(2011GXNSFA018080)。

摘　　要：以产漆酶的红芝LYL263菌株为试材,采用不同微生物混合培养的方法,研究了红芝LYL263与苏平平菇、白金金针菇、凤尾菇混合培养的产酶效果和产酶条件,以期能筛选出提高红芝LYL263产漆酶的相容菌株以及与之相适应的产酶条件。结果表明:红芝LYL263与其相容菌株-苏平平菇混合培养后漆酶活性达到1346.4 U·mL^(-1),比红芝LYL263单独培养产酶提高35.7%;红芝LYL263与苏平平菇的接种比例为4:2时,其产酶量最高,最优接种时间是同时接入红芝LYL263和苏平平菇。Taking laccase-producing LYL263 from Ganoderma lucidum as test material,the enzyme production effect and conditions of LYL263 with Flammulina velutipes,Pleutotus ostreatus and Pleurotus pulmonarius were studied by the mixed culture method of different microorganisms,in order to screen out compatible strains and adaptive conditions that could improve the laccase-producing capacity of LYL263.The results showed that laccase activity reached 1346.4 U·mL^(-1)after mixed culture of LYL263 and its compatible strain,Pleutotus ostreatus,which was 35.7%higher than that of LYL263 cultured alone.When the inoculation ratio of LYL263 and Pleutotus ostreatus was 4∶2,the enzyme production was the highest.The optimal inoculation time was to add LYL263 and Pleutotus ostreatus at the same time.

关 键 词：红芝LYL263 漆酶 混合培养 高产菌株筛选 

分 类 号：S567.31[农业科学—中草药栽培]