聚多巴胺涂覆的磺丁基醚-β-环糊精开管毛细管电色谱柱的制备及应用  

Preparation and application of polydopamine/sulfobutyl ether-βcyclodextrin coated open tube capillary electrochromatographic column

作　　者：黄子为 霍虹懿 龙科 石爽[1] 关瑾[1] HUANG Ziwei;HUO Hongyi;LONG Ke;SHI Shuang;GUAN Jin(College of Science,Shenyang University of Chemical Technology,Shenyang 110142,China)

机构地区：[1]沈阳化工大学理学院,沈阳110142

出　　处：《分析试验室》2024年第4期497-501,共5页Chinese Journal of Analysis Laboratory

基　　金：辽宁省自然科学基金(2019-ZD-0073)项目资助。

摘　　要：制备了以聚多巴胺/磺丁基醚-β-环糊精(PDA/SBE-β-CD)为手性固定相的开管毛细管电色谱柱。最佳制备条件为:SBE-β-CD浓度10.0 mg/mL,多巴胺浓度5.0 mg/mL,涂覆时间2 h。通过红外光谱和扫描电镜对制备的色谱柱进行了表征,并将其应用于奥美拉唑对映体的毛细管电色谱(CEC)分离分析。在110 mmol/L Tris-H_(3)PO_(4)(p H 6.0)缓冲溶液和外加电压15 k V条件下,奥美拉唑对映体在13 min内成功分离,分离度为5.82。对映异构体质量浓度在50~600μg/mL范围内线性关系良好(r^(2)=0.9958),迁移时间和峰面积的相对标准偏差(RSD)分别小于2.8%和4.8%。PDA/SBE-β-CD固定相在毛细管电色谱(CEC)手性分离中具有良好的应用潜力。An open tube capillary electrochromatographic column was prepared using polydopamine/sulfobutyl ether-β-Cyclodextrin(PDA/SBE-β-CD)as chiral stationary phase.The optimal conditions of fabricated capillary column were as follows:SBE-β-CD concentration of 10.0 mg/mL,dopamine concentration of 5.0 mg/mL,and coating time of 2 h.The fabricated capillary column was characterized by scanning electron microscopy and Fourier transform infrared spectroscopy.Furthermore,the chiral separation effectiveness of the fabricated capillary column was evaluated by capillary electrochromatography(CEC)using enantiomers of omeprazole as model analyte.The enantiomers of omeprazole were resolved within 13 min with high resolution of 5.82 using 110 mmol/L Tris-H_(3)PO_(4) buffer(pH 6.0)at applied voltage of 15 kV.Good linearities were obtained in the concentration range of 50-600μg/mL with the correlation coefficients of r ^(2)=0.9958 for the enantiomers of omeprazole.And for the migrated time and peak areas,the relative standard deviations(RSDs)were below 2.8%and 4.8%,respectively.The results reveal that PDA/SBE-β-CD has great potential as chiral stationary phases for enantio separation in CEC.

关 键 词：毛细管电色谱 手性分离 开管柱 磺丁基醚-Β-环糊精 聚多巴胺 

分 类 号：O657.8[理学—分析化学]