十六烷基三甲基溴化铵增敏化学发光法测定东莨菪内酯  

Determination of scopoletin by chemiluminescence with cetyltrimethylammo⁃nium bromide as sensitizer

作　　者：赵悦 张罗一览 程轲 向警 吴文婕 王琴 张雨润 黄文静 孙建敏[4] 杨宪 郑红 ZHAO Yue;ZHANG Luoyilan;CHENG Ke;XIANG Jing;WU Wenjie;WANG Qin;ZHANG Yurun;HUANG Wenjing;SUN Jianmin;YANG Xian;ZHENG Hong(College of Life Sciences,Chongqing Nor-mal University,Engineering Research Center of Active Matter Biotechnology,Ministry of Education,Chongqing 401331,China;College of Chemistry,Chongqing Normal University,Engineering Research Center of Active Matter Biotechnology,Ministry of Education,Chongqing 401331,China;Central Laboratory of Chongqing Water Conservancy Investment(Group)Company Limited,Chongqing 401100,China;Scientific Research Department of Chongqing Normal University,Chongqing 401100,China)

机构地区：[1]重庆师范大学生命科学学院,活性物质生物技术教育部工程研究中心,重庆401331 [2]重庆师范大学化学学院,活性物质生物技术教育部工程研究中心,重庆401331 [3]重庆市水利投资(集团)有限公司中心实验室,重庆401100 [4]重庆师范大学科研处,重庆401331

出　　处：《分析试验室》2024年第4期517-521,共5页Chinese Journal of Analysis Laboratory

基　　金：重庆市中央林业改革发展资金科技推广示范项目(渝林科推[2020年]10号);重庆市科学技术局基础研究与前沿探索项目(cstc2018jcyjA2390)资助。

摘　　要：本文提供了一种新的测定东莨菪内酯的化学发光方法。酸性条件下,十六烷基三甲基溴化铵(CTAB)可增强东莨菪内酯在高锰酸钾(K_(2)MnO_(4))-甲醛体系中的发光强度,因此以CTAB作为增敏剂和助溶剂,联合流动注射法对东莨菪内酯进行测定。在优化条件下,相对发光强度与东莨菪内酯浓度在0.1~26.0μmol/L范围内有良好的线性关系,检出限(3σ)达1.40 nmol/L。对2.0μmol/L东莨菪内酯连续测定11次,相对标准偏差(RSD)为1.1%。本方法可用于测定样品中东莨菪内酯的含量。A new chemiluminescence method for the determination of scopoletin was developed.Under acidic conditions,cetyltrimethylammonium bromide(CTAB)can enhance the luminescence intensity of scopoletin in the potassium permanganate-formaldehyde system.Therefore,using CTAB as sensitizer and co-solvent,scopoletin could be measured by combining chemiluminescence with flow injection method.Under the optimized conditions,the relative luminescence intensities showed good linearity with scopoletin concentrations in the range of 0.1-26.0µmol/L,and the detection limit(3σ)was 1.40 nmol/L.In addition,the relative standard deviation(RSD)was 1.1%for 11 consecutive determinations of 2.0µmol/L of scopoletin.The developed method can be applied to the determination of scopoletin in samples with satisfactory results.

关 键 词：东莨菪内酯 增敏 十六烷基三甲基溴化铵 流动注射 化学发光 

分 类 号：O657.3[理学—分析化学] R284.1[理学—化学]