液相色谱-串联质谱法测定植物源驱蚊防蚊产品中11种致敏芳香剂和3种化学驱避剂  

Determination of 11 allergic fragrances and 3 chemical repellents in plant derived mosquito repellent and anti mosquito products by liquid chromatography-tandem mass spectrometry

作　　者：胡玉霞 李红艳 陈德文 HU Yuxia;LI Hongyan;CHEN Dewen(Zhejiang Institute of Product Quality and Safety Science,Hangzhou 310018,China;Zhejiang Institute of Economics and Trade,Hangzhou 310018,China;Hangzhou Vocational&Technical College,Ecology and Health Institute,Hangzhou 310018,China)

机构地区：[1]浙江省产品质量安全科学研究院,杭州310018 [2]浙江经贸职业技术学院,杭州310018 [3]杭州职业技术学院生态健康学院,杭州310018

出　　处：《分析试验室》2024年第4期522-527,共6页Chinese Journal of Analysis Laboratory

基　　金：国家市场监管总局科技计划项目(2022MK047);浙江省市场监督管理局科技项目(20210102)资助。

摘　　要：建立了植物源驱蚊防蚊产品中11种致敏性芳香剂和3种化学驱避剂同时测定的液相色谱-串联质谱方法。样品用二氯甲烷超声提取,在电喷雾离子源(ESI)正负离子多反应监测模式下同时采集,以乙腈-水为流动相梯度洗脱,采用Fastcore C_(18)色谱柱进行分离,外标法定量。结果表明,该方法可以有效分离同分异构体6-甲基香豆素和7-甲基香豆素,14种目标化合物在0.4~20000μg/L范围内线性关系良好,相关系数R2>0.99,方法定量限为0.001~1.0 mg/kg。以定量限、 4倍定量限和10倍定量限3个水平进行加标实验,回收率为80.6%~118%,相对标准偏差(RSD)为1.1%~7.4%。采用本方法对30批驱蚊产品进行检测,检出香兰素、香豆素、避蚊胺和丁香酚4种目标物。该方法适用于同时检测植物源驱蚊产品中致敏性芳香剂和化学驱避剂。A liquid chromatography-tandem mass spectrometry(LC-MS/MS)method was developed for the determination of 11 allergic fragrances and 3 chemical repellents in plant derived mosquito repellent and anti mosquito products.After ultrasonic extraction with dichloromethane,the samples were analyzed simultaneously under electric spray ion source(ESI)positive and negative ion multi reaction monitoring mode(MRM).Chromatographic separation of the migration solution was performed on a Fastcore C_(18) chromatographic column by a gradient elution of acetonitrile and water.The external standard method was used for the quantitative analysis.The results showed that the isomers 6-methylcoumarin and 7-methylcoumarinthe could be separated efficiently.There were good linear relationships for the 14 targets in the concentration range of 0.4-20000µg/L,with the correlation coefficients higer than 0.99,and the method limits of quantification(LOQs)were 0.001-1.0 mg/kg.The recoveries of the targets were 80.6%-118%at three spiked levels of LOQ,4 LOQ and 10 LOQ.The relative standard deviations(RSDs)were 1.1%-7.4%.This method was used to detect 30 mosquito repellent products,and 4 targets including vanillin,coumarin,N,N-diethyl-meta-toluamide(DEET)and eugenol were detected.This method could be applied for the determination of allergenic fragrances and chemical repellents in plant derived mosquito repellent and anti mosquito products.

关 键 词：液相色谱-串联质谱法(LC-MS/MS) 致敏性芳香剂 化学驱避剂 驱蚊 

分 类 号：O657.63[理学—分析化学]