Metabolomic evidence of independent biotransformation pathways for terpenes in two specialist mammalian herbivores (genus Neotoma)  

作　　者：Katharina SCHRAMM Michele SKOPEC Denise DEARING 

机构地区：[1]Department of Biology,University of Utah,Salt Lake City,Utah,USA [2]Department of Zoology,Weber State University,Ogden,Utah,USA

出　　处：《Integrative Zoology》2024年第1期143-155,共13页整合动物学（英文版）

摘　　要：Herbivory is common in mammals,yet our understanding of detoxification processes used by mammals to biotrans-form plant secondary compounds(PSCs)is limited.Specialist herbivores are thought to have evolved detoxifica-tion mechanisms that rely more heavily on energetically cheap Phase I biotransformation reactions to process high levels of PSCs in their diets.We explored this hypothesis by comparing the urinary metabolite patterns of two spe-cialist herbivores(genus Neotoma).Neotoma stephensi is an obligate specialist on one-seeded juniper(Juniperus monosperma).Neotoma lepida is a generalist forager across its range,yet populations in the Great Basin specialize on Utah juniper(J.osteosperma).While both juniper species have high levels of terpenes,the terpene profiles and quantities differ between the two.Individuals from both woodrat species were fed diets of each juniper in a cross-over design.Urine,collected over a 24-h period,was extracted and analyzed in an untargeted metabolomics approach using both GC-MS and HPLC-MS/MS.The obligate specialist N.stephensi excreted a unique pattern of Phase I metabolites when fed its native juniper,while N.lepida excreted a unique pattern of Phase II metabolites when fed its native juniper.Both woodrat species utilized the Phase II metabolic pathway of glucuronidation more heavily when consuming the more chemically diverse J.osteosperma,and N.stephensi utilized less glucuronida-tion than N.lepida when consuming J.monosperma.These results are consistent with the hypothesis that obligate specialists may have evolved unique and efficient biotransformation mechanisms for dealing with PSCs in their diet.

关 键 词：dietary specialization HERBIVORY JUNIPER metabolomics NEOTOMA 

分 类 号：Q95[生物学—动物学]