Obese Ningxiang pig-derived microbiota rewires carnitine metabolism to promote muscle fatty acid deposition in lean DLY pigs  

作　　者：Jie Yin Yunxia Li Yu Tian Feng Zhou Jie Ma Siting Xia Tong Yang Libao Ma Qinghua Zeng Gang Liu Yulong Yin Xingguo Huang 

机构地区：[1]College of Animal Science and Technology,Hunan Agriculture University,Changsha 410128,China [2]College of Animal Sciences and Technology,Huazhong Agricultural University,Wuhan 430070,China [3]Hunan Chuweixiang Agriculture and Animal Husbandry Co,Ltd,Ningxiang 410600,China [4]Instiute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China

出　　处：《The Innovation》2023年第5期69-77,共9页创新（英文）

基　　金：National Natural Science Foundation of China(U20A2055 and 32172761);National Key Research and Development Program of China(2022YFD1301500);Hunan Youth Science and Technology Talent Project(2022RC1157).

摘　　要：The gut microbiota consistently shows strong correlations with lipid metabolism in humans and animals,and whether the gut microbiota contributes to muscle fatty acid(FA)deposition and meat traits in farm animals has not been fully resolved.In this study,we aimed to unveil the microbial mechanisms underlying muscle FA deposition in pigs.First,we systematically revealed the correlation between the gut microbiome and muscle FA levels in 43 obese Ningxiang pigs and 50 lean Duroc Landrace Yorkshire(DLY)pigs.Mutual fecal microbial transplantation showed that the obese Ningxiang pig-derived microbiota increased the muscle FA content and improved meat quality by reshaping the gut microbial composition in lean DLY pigs.Lactobacillus reuteri has been identified as a potential microbial biomarker in obese Ningxiang pig-derived microbiota-challenged DLY pigs.A gavage experiment using lean DLY pigs confirmed that L.reuteri XL0930 isolated from obese Ningxiang pigs was the causal species that increased the muscle FA content.Mechanistically,SLC22A5,a carnitine transporter,was downregulated in L.reuteri XL0930-fed DLY pigs,resulting in reduced muscle carnitine levels.Muscle and intestinal L-carnitine levels,which correlated with the muscle FA content,impeded fat synthesis and FA accumulation in in vitro and in vivo models.In conclusion,we uncovered an unexpected and important role of the obese Ningxiang pig-derived microbiota in regulating muscle FA metabolism via the SLC22A5-mediated carnitine system.

关 键 词：DLY Ningxia fir 

分 类 号：S828[农业科学—畜牧学]