水位变动对三峡水库消落带典型雌激素效应物质及活性的影响  

The effects of water fluctuation on typical estrogens and activities in the water level fluctuation zone of the Three Gorges Reservoir

作　　者：周敏 邵迎 黄思瑜 陈忠礼 ZHOU Min;SHAO Ying;HUANG Siyu;CHEN Zhongi(Key Laboratory of the Three Gorges Reservoir Region’s Eco-Environment,Chongqing University,Chongqing 400044,P.R.China;Chongqing Institute for Food and Drug Control,Chongqing 401121,P.R.China)

机构地区：[1]重庆大学三峡库区生态环境教育部重点实验室,重庆400044 [2]重庆食品药品检测研究院,重庆401121

出　　处：《重庆大学学报》2024年第4期1-11,共11页Journal of Chongqing University

基　　金：国家自然科学基金资助项目(51909015)。

摘　　要：以三峡库区消落带为研究对象,将化学分析与生物测试相结合,研究水位变动对消落带土壤(落干期)和沉积物(淹水期)雌激素效应物质种类和含量及雌激素活性的影响,探讨典型雌激素与雌激素效应的关联程度,以期为水库消落带生态安全和环境健康管理提供重要的数据支撑。借助超高效液相色谱-质谱(UPLC-MS)对8种典型雌激素的赋存与质量浓度进行靶向分析,并使用重组基因酵母筛选(YES)体系检测环境样品雌激素活性,通过相关性分析和浓度加和计算建立化学物质与生物效应间的相关联系。8种典型雌激素仅有雌酮(E1)和乙炔基雌二醇(EE2)被检出,含量在0.025~2.667 ng/g范围内。淹水期的沉积物具有明显的雌激素活性,其雌二醇当量(EEQ)值为0.637~6.987 ng/g。相关性分析结果显示,靶向分析的雌激素效应物质与雌激素效应间无明显相关性,仅能解释29.46%的雌激素活性。水位变动影响消落带雌激素效应物质的种类和质量浓度,淹水提高了沉积物雌激素活性。典型雌激素物质与库区消落带雌激素效应无显著关联。因此,需要开发新的方法与技术,以便更为精确地指导消落带雌激素效应的风险识别与管控。In this study,the water level fluctuation zone of the Three Gorges Reservoir(TGR)was selected for investigation.A combination of chemical analysis and biological testing was utilized to investigate the effects of water fluctuation on the compounds and concentrations of estrogenic effector substances,as well as the estrogenic activity in soil(during the non-flooding period)and sediment(during the flooding period).The correlation between typical estrogens and estrogenic activity was analyzed.The study aimed to provide crucial data support for reservoir ecological safety and environmental health management.The distribution and concentration of 8 typical estrogens were analyzed using an ultra-performance liquid chromatography-mass spectrometry(UPLC-MS),while estrogenic activity was determined via the yeast estrogen screening assay(YES).Correlation analysis and concentration summation calculations were used to establish the relationships between chemical substances and biological effects.Among the 8 studied estrogens,only estrone(E1)and ethinylestradiol(EE2)were detected,with concentrations ranging from 0.025 ng/g to 2.667 ng/g.Sediments during the flooding period showed significantly higher estrogenic activity,with 17β-estradiol equivalents(EEQ)ranging from 0.637 ng/g to 6.987 ng/g.However,correlation analysis did not reveal a distinct correlation between the target compounds and estrogenic effects,as the detected chemicals only accounted for about 29.46%of the estrogenic activity.The results suggest that water fluctuation can influence the type and the concentration of estrogens in the water level fluctuation zone of the TGR,leading to increased estrogenic activity during the flooding period.The direct linkage between typical estrogens and estrogenic activity was not identified.Therefore,new methods and techniques are required to enhance identification accuracy and improve the management of the risk of estrogenic effects in the water level fluctuation zone.

关 键 词：沉积物 水位变动 三峡水库 雌激素活性 典型雌激素 

分 类 号：X524[环境科学与工程—环境工程]