柞蚕蛹虫草不同培养时间中的活性物质代谢组分析  

Metabolic Analysis of Active Substances in Different Culture Times of Cordyceps militaris cultivated with Antherea pernyi

作　　者：李亚洁 温志新[1,2] 孟楠 李学军[1,2] 陈星合 王林美[1,2] 都兴范 LI Yajie;WEN Zhixin;MENG Nan;LI Xuejun;CHEN Xinghe;WANG Linmei;DU Xingfan(Liaoning Ocean and Fishery Science Research Institute,Dalian 116023,China;Dalian Institute of Biotechnology,Liaoning Academy of Agricultural Sciences/Liaoning Tussah Resources Deep Processing and Utilization Engineering Technology Research Center,Dalian 116024,China)

机构地区：[1]辽宁省海洋水产科学研究院,辽宁大连116023 [2]辽宁省农业科学院大连生物技术研究所/辽宁省柞蚕资源深加工利用工程技术研究中心,辽宁大连116024

出　　处：《中国食用菌》2024年第2期95-99,109,共6页Edible Fungi of China

基　　金：现代农业产业技术体系建设专项(CARS-18);辽宁省民生科技联合计划项目(2021JH2/10200039);辽宁省农业科学院重点学科资助项目(2022DD268646)。

摘　　要：为解析柞蚕蛹虫草不同培养时间中活性代谢物的种类及积累规律,运用广泛靶向代谢组学技术对培养0、10、20、30、40 d的柞蚕蛹虫草中的代谢物进行鉴定。结果表明,柞蚕蛹虫草不同培养时间中的主要活性物质种类大体相同,在各试验培养期中分别鉴定出419、419、420、417和417个代谢物,主要包括氨基酸及其衍生物、核苷酸及其衍生物、酚酸、有机酸、糖及醇类、脂类、生物碱等多种化合物。其中,首次检测到3-吲哚丙酸、吡哆素。柞蚕蛹虫草培养过程中差异代谢物的相对含量差异显著,所有的差异代谢物聚为9个簇,大致分为5种类型,其中,有61个代谢物在试验期内呈现持续上调趋势,包括虫草素、2-脱氧腺苷等。对比主要活性物质中6种核苷类成分及鲜味氨基酸含量,培养40 d的柞蚕蛹虫草品质更佳。To analyze the types and accumulation patterns of active metabolites in Cordyceps militaris cultivated with Antherea pernyi cultured at different times,extensive targeted metabolomics techniques were used to identify metabolites in C.militaris culvitated with A.pernyi cultured at 0,10,20,30 and 40 days.The results showed that the main types of active substances in C.militaris cultivated with A.pernyi cultured at different times were generally the same.In each experimental culture period,419,419,420,417 and 417 metabolites were identified,mainly including amino acids and their derivatives,nucleotides and their derivatives,phenolic acids,organic acids,sugars and alcohols,lipids,alkaloids and other compounds.Among them,3-indole and propionic acid and pyridoxine were detected for the first time.The relative content of differential metabolites during the cultivation process of C.militaris cultivated with A.pernyi showed significant differences.All differential metabolites were clustered into 9 clusters and roughly divided into 5 types.Among them,61 metabolites showed a continuous upregulation trend during testing time,including cordycepin and 2-deoxyadenosine.Comparing the content of 6 nucleoside components and fresh amino acids in the main active substances,the quality of C.militaris cultivated with A.pernyi cultured for 40 days is better.

关 键 词：柞蚕蛹虫草 活性物质 代谢 

分 类 号：S646.9[农业科学—蔬菜学]