棘皮动物海星(Patiria miniata)全基因组微卫星分布特征  

Characteristics of Microsatellites Distribution in the Whole Genome of Patiria miniata(Echinodermata)

作　　者：曾志锋 邵伟伟[2] 马力 陶宇慧 韦力[2] ZENG Zhifeng;SHAO Weiwei;MA Li;TAO Yuhui;WEI Li(Guilin Forestry and Landscaping Integrated Service Centre,Guilin 541199,Guangxi;School of Ecology,Lishui University,Lishui 323000,Zhejiang;Jinyun Forestry Bureau,Jinyun 321400,Zhejiang)

机构地区：[1]桂林市林业和园林综合服务中心,广西桂林541199 [2]丽水学院生态学院,浙江丽水323000 [3]缙云县林业局,浙江缙云321400

出　　处：《丽水学院学报》2024年第2期31-39,共9页Journal of Lishui University

基　　金：浙江省自然科学基金资助项目“低温延长宁波滑蜥卵滞留及其分子机制”(LQ20C030001)。

摘　　要：微卫星存在于大多数生物体中。本研究采用生物信息学方法对棘皮动物门的海星全基因组微卫星分布规律进行分析。结果表明:海星全基因组微卫星总数为163 819个,总长度为2 655 030 bp,占基因组序列总长度的0.44%。微卫星单碱基、二碱基、三碱基、四碱基、五碱基和六碱基重复单元中数量最多的分别为(G)_(n)、(AT)_(n)、(TTA)_(n)、(TTTG)_(n)、(ACTCG)_(n)和(TACATG)_(n)。海星不同类型微卫星的重复次数分布差异较大。不同基因区和基因间区的数量及丰度不同,其中基因间区的微卫星数量及其丰度最大,为82 871个(3 229.00个/Mb),编码区的微卫星数量及其丰度最小,为936个(226.29个/Mb)。编码区中,微卫星最多的类型为三碱基重复,非翻译区、外显子、内含子和基因间区最多的类型均为单碱基重复。在微卫星丰度分布的位置特征分析中,基因上游500 bp和下游500 bp区域的微卫星丰度最大,分别为4 527.64个/Mb和4 844.82个/Mb。在外显子中,倒数第一外显子的丰度最大(数量为2 686个),为1 725.52个/Mb,其余外显子的丰度较为接近。不同位置区域的内含子丰度也较为接近,丰度为2 730.42~3 047.94个/Mb。本研究结果为海星的进一步深入研究提供参考数据。Microsatellites are found in most living organisms.In this present study,the distribution o microsatellites in the complete genome of Patiria miniata(Echinodermata)was analyzed using bioinformatics method.The results showed that there were 163 819 microsatellites in the whole genome of P.miniata,with a total length of 2 655 030 bp,accounting for 0.44%of the total length of genome sequence.The microsatellite mononucleotide,dinucleotide,trinucleotide,tetranucleotide,pentanucleotide and hexanucleotide that contained the largest number of repeats were(G)_n,(AT)_n,(TTA)_n,(TTTG)_n,(ACTCG)_n,and(TACATG)_(n )respectively.The repeat distribution of microsatellites differed greatly in types of P.miniata.The number and abundance of microsatellites varied in gene regions and intergenic regions.The number and abundance of microsatellites in intergenic regions were the highest.There were 82 871 microsatellites,with the abundance of3 229.00 loci/Mb.The number and abundance of microsatellites in the coding region were the lowest.There were936 microsatellites,with the abundance of 226.29 loci/Mb.Trinucleotide repeats were the most abundant microsatellites in the coding region,while mononucleotide repeats were the most abundant microsatellites in the untranslated regions,exons,introns and intergenic regions.In the analysis of location characteristics of abundance distribution of microsatellites,the abundances of microsatellites in the regions of 500 bp upstream and downstream of genes were the highest,which were 4 527.64 loci/Mb and 4 844.82 loci/Mb respectively.In exons the abundance of the tailender was the highest.There were 2 686 microsatellites,with the abundance of1 725.52 loci/Mb;the abundances of the other exons were similar.The abundances of introns in different regions were similar too,ranging from 2 730.42 loci/Mb to 3 047.94 loci/Mb.We hope these results will provide reference data for further investigation of P.miniata.

关 键 词：棘皮动物门 海星 全基因组 微卫星 分布规律 

分 类 号：Q959.1[生物学—动物学]