基于微流控芯片-间接电位法的氧化三甲胺分析方法  

An analytical method for trimethylamine oxide based on microfluidic chip-indirect potentiometric method

作　　者：费家裕 袁宁 王孟歌 陈小锋[1] 李明[1] FEI Jiayu;YUAN Ning;WANG Mengge;CHEN Xiaofeng;LI Ming(School of Environmental Science and Engineering,Yangzhou University,Yangzhou 225127,China)

机构地区：[1]扬州大学环境科学与工程学院,江苏扬州225127

出　　处：《扬州大学学报（自然科学版）》2024年第2期35-41,共7页Journal of Yangzhou University：Natural Science Edition

基　　金：国家自然科学基金资助项目(42177185);浙江省舟山市疾病预防控制中心海产品健康危害因素关键技术研究重点实验室开放课题资助项目(202304)。

摘　　要：为了实现海产品体内氧化三甲胺(trimethylamineoxide,TMAO)的快速定量检测,提出一种采用微流控光化学还原芯片进行前处理的电化学分析方法.首先构建了PDMS/SiO_(2)微流控芯片,并对TMAO的前处理反应条件进行了优化:采用该微流控芯片,使用Fe(Ⅱ)-HEDP为还原剂,在pH值为6.5,温度为40℃,紫外光照时间为2min的条件下,TMAO被还原为三甲胺(trimethylamine,TMA)的还原率达99.44%;然后,利用自制选择性电极对TMA溶液进行检测,在0.01~10mmol·L^(-1)范围内,TMA浓度对数与其溶液电位值线性相关,相关系数r^(2)=0.9977,检测限为2.28μmol·L^(-1).采用该方法对实际海鲜产品进行TMAO检测的相对偏差不超过4.71%,加标回收率为91.78%~112.16%.该方法测定时间短、结果准确,在海产品分析检测中具有良好的应用前景。Aiming at the rapid quantitative detection of trimethylamine oxide(TMAO)in seafood,an electrochemical analysis method using microfluidic photochemical reduction chip for pretreatment is proposed.Firstly,the PDMS/SiO,hybrid microfluidic chip is fabricated,and the pre-treatment reaction reduction conditions of TMAO are optimized.Using this microfluidic chip and Fe(Ⅱ)-HEDP as reducing agent,under the conditions of pH 6.5,temperature 40'C and UV-irradiation time 2 min,the reduction rate of TMAO to trimethylamine(TMA)can reach 99.44%.Then,the self-made selective electrode is used to detect TMA solution.In the range of 0.01~10 mmol·L^(-1),the logarithm of TMA concentration is linearly correlated with the solution potential value,the cor-relation coefficient r^(2) is 0.9977,and the detection limit is 2.28μmol·L^(-1).The relative deviation of this method for TMAO detection of actual seafood products is not more than 4.71%,and the recovery rate is 91.78%~112.16%.The method has the advantages of short determination time and accurate results,and shows a good application prospect in the analysis and detection of seafood.

关 键 词：微流控芯片 样品前处理 氧化三甲胺 三甲胺 光化学还原 

分 类 号：O657.15[理学—分析化学]