花生GATA基因家族鉴定和表达分析  

Identification and expression analysis of peanut GATA gene family

作　　者：王伟杰 雷亚柯 张建航 展世杰 邓陈威 贾朝阳 Wang Weijie;Lei Yake;Zhang Jianhang;Zhan Shijie;Deng Chenwei;Jia Chaoyang(Zhoukou Academy of Agricutural Science,Zhoukou 466000,China)

机构地区：[1]周口市农业科学院,河南周口466000

出　　处：《山西农业大学学报（自然科学版）》2024年第2期10-22,共13页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：河南省科研平台22年产业基金(225101610063);河南省优质专用型花生工程技术研究中心(20221108038)。

摘　　要：[目的]探究GATA基因家族在花生生长发育和抗旱调控中的作用。[方法]通过生物信息学方法鉴定花生GATA家族成员,分析其系统进化、基因结构、组织表达模式,最后结合转录组数据和实时荧光定量PCR技术筛选花生GATA家族响应干旱胁迫的关键基因。[结果]本文鉴定到47个GATA类转录因子编码基因,它们不均匀地分布在除2号、4号和14号染色体以外的17条染色体上。理化性质分析表明,该家族属于亲水性蛋白,大部分基因呈酸性,只有Arahy.QG9XFC.1具有信号肽。根据拟南芥家族同源基因聚类分析结果,可将花生GATA家族蛋白分为4个亚家族。其中第Ⅰ、Ⅱ、Ⅲ亚家族成员的锌指结构域特征模体为C_(X2)C_(X18)C_(X2)C,而第Ⅳ亚家族为C_(X2)C_(X20)C_(X2)C类型。大部分成员在A亚基因组和B亚基因组中呈对称分布,组织表达模式分析显示,超过半数的GATA家族成员在22个组织中表现不同程度的特异性表达。其中第Ⅰ亚家族Arahy.LJYJ4M、Arahy.VS8GG1、Arahy.3S8P0L和Arahy.I6JZDT在所有组织中均显示较高的表达水平。然而Arahy.QY7BN7和Arahy.F7WS4I在叶片,Arahy.C6NV9N在花被中表达较高,Arahy.4Y7J59和Arahy.D56WMI在种子中呈现组织特异性高表达特性。转录组数据分析发现,34个GA⁃TA基因响应干旱、低温、ABA和BR激素处理,5个GATA基因在干旱和低温胁迫下表达量发生显著上调,1个基因发生显著下调。进一步RT-qPCR分析表明,Arahy.C6NV9N和Arahy.2SUK7S特异性响应PEG胁迫的基因,尤其前者在叶组织中胁迫早期呈现持续上调表达。[结论]花生中有47个GATA基因,其中Arahy.C6NV9N和Ara⁃hy.2SUK7S可能是花生GATA家族响应干旱胁迫关键基因。[Objective]This study aimed to investigate the roles of the GATA gene family in the growth,development,and drought resistance regulation of peanuts.[Methods]The peanut GATA family members were identified using bioinformatics methods,and their systematic evolution,gene structure,and tissue expression patterns were analyzed.Finally,key genes responsive to drought stress in the peanut GATA family were screened by integrating transcriptome data and real-time fluorescence quantitative PCR technology.[Results]In this study,47 GATA transcription factor encoding genes were identified,unevenly distributed on 17 chromosomes except for chromosomes 2,4,and 14.Physicochemical property analysis showed that this family belongs to hydrophilic proteins,with most genes being acidic and only Arahy.QG9XFC.1 possessing a signal peptide.Based on homologous gene clustering analysis with Arabidopsis family,peanut GATA family proteins were classified into four subfamilies.The zinc finger structure characteristic motif of members in subfamilyⅠ,Ⅱ,andⅢwere C_(X2)C_(X18)C_(X2)C,while subfamilyⅣwas C_(X2)C_(X20)C_(X2)C type.Most members were symmetrical distributed in A and B subgenomes.Tissue expression pattern analysis showed that over half of the GATA family members showed varying degrees of tissue-specific expression among 22 tissues.Among them,members,Arahy.LJYJ4M,Arahy.VS8GG1,Arahy.3S8P0L,and Arahy.I6JZDT of subfamily I showed high expression levels in all tissues.However,Arahy.QY7BN7 and Arahy.F7WS4I showed higher expression in leaves,Arahy.C6NV9N in petals,and Arahy.4Y7J59 and Arahy.D56WMI showed tissue-specific high expression in seeds.Transcriptome data analysis revealed that 34 GATA genes responded to drought,low temperature,ABA,and BR hormone treatments,with five GATA genes significantly up-regulated under drought and low-temperature stress,and one gene significantly down-regulated.Further RT-qPCR analysis showed that Arahy.C6NV9N and Arahy.2SUK7S were specifically responsive to PEG stress,especially with the former showin

关 键 词：花生 转录因子 GATA 组织表达模式 干旱胁迫 

分 类 号：S565.2[农业科学—作物学]