白僵菌在大豆植株中的内生性及对八字地老虎幼虫杀虫活性研究  

Endogeneity of Beauveria bassiana in soybean plants and its insecticidal activity against larva of Xestia c-nigrum

作　　者：刘晓丽 张鑫鑫[1] 于军华 李玥玥 于洪春[1] LIU Xiaoli;ZHANG Xinxin;YU Junhua;LI Yueyue;YU Hongchun(School of Plant Protection,Northeast Agricultural University,Harbin 150030,China;Agricultural Development Department,Hongxinglong Branch,Beidahuang Group Co.,Ltd.,Youyi Heilongjiang 155811,China)

机构地区：[1]东北农业大学植物保护学院,哈尔滨150030 [2]北大荒集团有限公司红兴隆分公司农业发展部,黑龙江友谊县155811

出　　处：《东北农业大学学报》2024年第1期14-22,共9页Journal of Northeast Agricultural University

基　　金：国家重点研发计划项目(2022YFF1300504);黑龙江省应用技术研究与开发计划重大项目(GA19B104)。

摘　　要：研究球孢白僵菌Bb-170428菌株大豆种子包衣处理后大豆植株内生性、不同组织部位定殖率和宿存量,以及包衣处理植株对八字地老虎幼虫杀虫活性和生长发育的影响。结果表明,白僵菌Bb-170428菌株在大豆植株根、茎、叶中均成功定殖,根部、下叶、下茎定殖率较高,上茎和上叶定殖率较低,下茎和下叶宿存量显著高于上叶、上茎和根部,定殖率和宿存量均在播种后14 d达到峰值,随后呈下降趋势。白僵菌种子包衣处理对大豆植株生长具有明显促生作用,植株对八字地老虎幼虫具有杀虫活性,饲喂18 d后,1~4龄幼虫校正死亡率分别达到53.25%、37.97%、27.50%和23.75%。将白僵菌种子包衣处理的植株饲喂5龄幼虫,幼虫死亡率和蛾畸形率显著升高,化蛹率、羽化率和蛹重显著降低,不利于高龄幼虫生长发育。研究结果为白僵菌生物种衣剂的开发应用提供理论和试验依据,增加了地老虎生物防治新途径,对绿色食品生产及减少化学农药使用具有重要意义和实际价值。The endogeneity of Beauveria bassiana Bb-170428 in soybean plants after seed coating,the colonization rate and number in different parts,and the effect of coated plants on the insecticidal activity and growth and development of the larvae of the Xestia c-nigrum were analyed.The results showed that Bb-170428 strains of Beauveria bassiana were successfully colonized in the roots,stems and leaves of soybean plants.The colonization rate was higher in the roots,lower leaves and lower stems,and lower in the upper stems and upper leaves.The colonization number of the lower stems and lower leaves was significantly higher than that of the upper leaves,upper stems and root,the colonization number of lower stems and leaves was significantly greater than that of upper leaves,upper stems and roots.Both the colonization rate and the number reached the peak at 14 d after seeding,and then showed a downward trend.The seed coating treatment of Beauveria bassiana had a significant effect on the growth of soybean plants,and the plants had insecticidal activity against the larva of the Xestia c-nigrum.After feeding for 18 d,the corrected mortality rates of the 1-4 instar larvae reached 53.25%,37.97%,27.50%and 23.75%,respectively.Plants coated with Beauveria bassiana seed were fed with 5th instar larvae,the larval mortality rate and moth malformation rate increased significantly,while the pupation rate,emergence rate and pupa weight decreased significantly,which had adverse effects on the growth and development of elderly larvae.The research results provide theoretical and experimental basis for the development and application of biological-coating agent of Beauveria bassiana,increase a new way of biological control of cutworm,and have important significance and practical value for green food production and reducing the use of chemical pesticides.

关 键 词：白僵菌 八字地老虎 内生性 促生 杀虫活性 

分 类 号：S435.112.1[农业科学—农业昆虫与害虫防治]