3种N-DBPs对青海弧菌Q67的联合毒性作用及贡献度  

Combined toxicity interactions and contribution of three N-DBPs to Vibrio qinghaiensis sp.-Q67

作　　者：桂一心 张瑾 张颖 曾健平 陈如荔 GUI Yi-xin;ZHANG Jin;ZHANG Ying;ZENG Jian-ping;CHEN Ru-li(College of Environment and Energy Engineering,Anhui Jianzhu University,Hefei 230601,China;Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province,Hefei 230601,China)

机构地区：[1]安徽建筑大学环境与能源工程学院,安徽合肥230601 [2]安徽省水污染控制与废水资源化重点实验室,安徽合肥230601

出　　处：《中国环境科学》2024年第5期2742-2753,共12页China Environmental Science

基　　金：国家重点研发计划项目子课题(2021YFC3201005);安徽省教育厅创新团队项目(2022AH010019);环境污染控制与废弃物资源化利用安徽省重点实验室开放课题(2023EPC07)。

摘　　要：以3种含氮消毒副产物(N-DBPs)溴乙腈(BAN)、二溴乙腈(DBAN)和二氯乙酰胺(DCAM)为研究对象,运用直接均分和均匀设计射线法分别设计了3个二元混合物体系(BAN-DBAN、BAN-DCAM、DBAN-DCAM)和1个三元混合物体系(BAN-DBAN-DCAM),采用时间毒性微板分析法(t-MTA)系统测定3种N-DBPs及其混合物体系对青海弧菌(Q67)的毒性,应用浓度加和模型(CA)分析混合物的毒性相互作用,并采用剂量减小指数(DRI)来表征混合物中各个组分对毒性相互作用的贡献情况.结果表明,BAN、DBAN和DCAM对Q67的浓度效应曲线为“S”型,具有明显的急性毒性,长期毒性均高于急性毒性(BAN-pEC_(50)-0.25h=4.16<BAN-pEC_(50)-12h=4.93;DBAN--pEC_(50)-0.25h=4.36<BAN-pEC_(50)-12h=4.84;DBAN--pEC_(50)-0.25h=1.41<BAN-pEC_(50)-12h=1.61);BAN-DCAM混合物出现拮抗作用,BAN-DBAN出现协同作用,而DBAN-DCAM与BAN-DBAN-DCAM既出现拮抗作用又出现协同作用;3个二元混合物体系,除BAN-DCAM外,暴露初期均表现为不同强度的协同作用;在BAN-DBAN-DCAM体系中,DRI_(max,DBAN)(=258.706)>DRI_(max,BAN)(=38.166)>DRI_(max,DCAM)(=2.658),故推测3种组分对协同作用的贡献度为:DCAM>DBAN>BAN.Three nitrogenous disinfection by-products(N-DBPs),bromoacetonitrile(BAN),dibromoacetonitrile(DBAN)and dichloroacetamide(Dichloroacetamide,DCAM),were selected as research objects,and three binary mixture systems(BAN-DBAN,BAN-DCAM,DBAN-DCAM)and one ternary mixture system(BAN-DBAN-DCAM)were designed by the direct equipartition and uniform design ray methods,respectively.The time-dependent microplate toxicity analysis(t-MTA)was used to systematically determine the toxicity of the three N-DBPs and their mixture systems against Vibrio qinghaiensis sp.-Q67,Q67.A concentration addition(CA)model was applied to analyze toxicity interactions within mixtures,and the dose reduction index(DRI)method was used to characterize the contribution of individual components to the combined toxicity interactions.The results showed that the concentration-effect curves of BAN,DBAN and DCAM on Q67 were"S"-shaped with obvious acute toxicity,and the long-term toxicity was higher than the acute toxicity.BAN-DCAM mixture showed antagonism,the BAN-DBAN showed synergism,while the DBAN-DCAM and BAN-DBAN-DCAM appeared both antagonism and synergism.The three binary mixture systems,except BAN-DCAM,showed synergistic effects with different intensities at the early stage of exposure.In the BAN-DBAN-DCAM system,DRImax,DBAN(=258.706)>DRImax,BAN(=38.166)>DRImax,DCAM(=2.658),so the contribution degree of the three N-DBPs to the synergistic effects was presumed to be followed by DCAM>DBAN>BAN.

关 键 词：含氮消毒副产物 青海弧菌Q67 联合毒性 相互作用 剂量减小指数 

分 类 号：X503.2[环境科学与工程—环境工程]