激素对圆齿野鸦椿种子萌发的影响  

Effects of hormones on seed germination of Euscaphis konishii

作　　者：阳先波 冉芳 周宇曦 涂淑萍[1] 蔡军火[1] 姜雪茹 钟鑫宏 YANG Xian-bo;RAN Fang;ZHOU Yu-xi;TU Shu-ping;CAI Jun-huo;JIANG Xue-ru;ZHONG Xin-hong(College of Landscape and Art,Jiangxi Agricultural University,Nanchang,Jiangxi 330045,China)

机构地区：[1]江西农业大学园林与艺术学院,江西南昌330045

出　　处：《南方农业学报》2024年第1期160-168,共9页Journal of Southern Agriculture

基　　金：国家自然科学基金项目(31960327);江西省自然科学基金项目(20212BAB215014)。

摘　　要：【目的】探究激素在圆齿野鸦椿种子萌发中的作用及其与活性氧的关系,为圆齿野鸦椿种子破眠技术和种苗高效繁育技术提供理论依据。【方法】以自然层积210 d的圆齿野鸦椿种子为试验材料,去除外部硬质种壳,进行为期12 d的萌发试验,测定纯水、不同浓度的赤霉素(GA_(3))(20、40和60μmol/L)和脱落酸(ABA)(10、20和30μmol/L)处理下萌发率及活性氧、抗氧化物质、植物激素、可溶性糖和可溶性蛋白含量的变化,并观察记录种子的萌发形态。【结果】纯水处理萌发率最终达77.8%,GA_(3)处理萌发率最高可达82.2%,ABA处理萌发率最终均未达50.0%,而保留硬质种皮纯水处理的种子萌发率仅17.8%。3种浓度的GA_(3)处理均可有效缩短圆齿野鸦椿种子萌发率达50.0%的时间(T_(50)),促进根茎生长,但其最终萌发率均与纯水处理无显著差异(P>0.05);ABA处理浓度越高,对圆齿野鸦椿种子抑制萌发作用越强。相关分析显示,在萌发的第8和12 d,萌发率与GA_(3)、油菜素内酯(BR)和茉莉酸(JA)呈显著正相关(P<0.05,下同),且超氧阴离子(O_(2)^(-)·)与BR和JA含量呈显著负相关,与过氧化氢酶(CAT)、ABA、ABA/GA_(3)均呈显著正相关。【结论】添加GA_(3)可有效缩短圆齿野鸦椿种子萌发率达50%的时间,促进根茎生长,O_(2)^(-)·与ABA的积累对种子萌发存在抑制作用,BR和JA对种子萌发的促进作用可能与对O_(2)^(-)·的清除有关。【Objective】The role of hormones in seed germination of Euscaphis konishii and its relationship with reac-tive oxygen species were explored to provide an important theoretical basis for its seed dormancy breaking technology and efficient seedling breeding technology.【Method】E.konishii seeds with 210 d natural stratified were used as materials,outer hard seed coat was removed,a 12 d germination experiment was conducted.The changes of germination rate,active oxygen,antioxidant substances,plant hormones,soluble sugar and soluble protein contens under pure water,different con-centrations of gibberellin(GA_(3))(20,40 and 60μmol/L)and abscisic acid(ABA)(10,20 and 30μmol/L)were measured,and germination morphology of seeds was observed.【Result】The germination rate of pure water treatment was 77.8%,the germination rate of GA_(3) treatment was up to 82.2%,the germination rate of ABA treatment did not reach 50.0%,and the germination rate of pure water treatment with hard seed coat was only 17.8%.The three concentrations of GA_(3) treat-ments could effectively shorten the time when germination rate reached 50.0%(T_(50))and promote root and stem growth,but the final germination rate was not significantly different from that of pure water treatment(P>0.05).ABA treatment could effectively inhibit the germination of E.konishii seeds.The higher the concentration of ABA,the stronger the inhibi-tion.Correlation analysis showed that the germination rate was significantly positively correlated with GA_(3),brassinolide(BR)and jasmonic(JA)at 8 and 12 d(P<0.05,the same below),superoxide anion(O_(2)^(-)·)was negatively correlated with BR and JA contents,and significantly positively correlated with catalase(CAT),ABA,ABA/GA_(3).【Conclusion】Adding GA_(3) can effectively shorten the time of the seed germination rate of E.konishii to reach 50%and promote the growth of root and stem.The accumulation of O_(2)^(-)·and ABA inhibit seed germination and the promotion of BR and JA on seed germination in E.konishii may be related t

关 键 词：圆齿野鸦椿 种子萌发 植物激素 活性氧 

分 类 号：S792.99[农业科学—林木遗传育种]