广西H9N2亚型禽流感病毒疫苗候选株的筛选  

10Screening of vaccine candidate strains of H9N2 subtype avian influenza virus in Guangxi

作　　者：李孟[1] 谢芝勋[1] 李丹[1] 徐倩[1] 罗思思[1] 张民秀[1] 谢丽基[1] 黄超 沈前程 黄娇玲 LI Meng;XIE Zhi-xun;LI Dan;XU Qian;LUO Si-si;ZHANG Min-xiu;XIE Li-ji;HUANG Chao;SHEN Qian-cheng;HUANG Jiao-ling(Guangxi Veterinary Research Institute/Guangxi Key Laboratory of Veterinary Biotechnology/Key Laboratory of China(Guangxi)-ASEAN Cross-border Animal Disease Prevention and Control,Ministry of Agriculture and Rural Affairs,Nanning,Guangxi 530001,China;Guangxi Jinling Agriculture and Animal Husbandry Group Company Limited,Nanning,Guangxi 530049,China)

机构地区：[1]广西兽医研究所/广西兽医生物技术重点实验室/农业农村部中国—东盟(广西)跨境动物疫病防控重点实验室,广西南宁530001 [2]广西金陵农牧集团有限公司,广西南宁530049

出　　处：《南方农业学报》2024年第1期243-252,共10页Journal of Southern Agriculture

基　　金：广西重点研发计划项目(桂科AB21076004);广西自然科学基金项目(2022GXNSFAA035445,2021GXNSFBA196031);广西八桂学者专项(2019-A50)。

摘　　要：【目的】筛选出免疫原性更优的H9N2亚型禽流感病毒(AIV)疫苗候选株用于新型疫苗研发,为加强对H9N2亚型AIV的防控提供技术支持。【方法】以2000—2020年广西地区分离获得的36株H9N2亚型AIV分离株为试验材料,依据HA基因遗传进化分析选择12株不同年份、不同地域及不同宿主来源的H9N2亚型AIV代表株,与目前使用的商品化H9亚型AIV疫苗株(SS株)进行交叉血凝抑制试验(HI)及抗原性分析,根据抗原分析结果选择其中具有不同抗原差异的代表株制备油乳剂灭活疫苗,并分别与商品化H9亚型AIV疫苗(SS株)进行交叉免疫保护试验。【结果】在36株H9N2亚型AIV广西分离株中有31株属于Y280-Like(9.4.1),其中26株属于分支I、5株属于分支II,另外5株属于G1-Like(h9.4.1),与我国常用疫苗株分属于不同进化分支。12株H9N2亚型AIV广西代表性分离株灭活后的HA效价≥7 log2,病毒灭活效果良好,可用于制备油乳剂灭活疫苗,且制备的油乳剂灭活疫苗稳定性和安全性良好。根据交互HI抗体滴度计算出各毒株间的抗原相关值(R),结果发现A/chicken/Guangxi/C227/2015(H9N2)株(简称C227株)与其他毒株的抗原性无明显差异,对应的R在0.72~0.93间波动;疫苗交叉保护试验结果也表明,C227株制备的油乳剂灭活疫苗对不同毒株的免疫保护率均高于80%,且免疫保护效果优于A/chicken/Guangxi/CX/2013(H9N2)株(简称CX13株),说明C227株更适合作为H9N2亚型AIV灭活疫苗的候选株。【结论】基于抗原性分析和免疫原性测定筛选出的A/chicken/Guangxi/C227/2015(H9N2)株对广西地区绝大部分H9N2亚型AIV流行株的免疫保护效果良好,可作为疫苗候选株用于研制新型H9N2亚型AIV疫苗。【Objective】The vaccine candidate strains of H9N2 subtype avian influenza virus(AIV)with better immu-nogenicity were screened for new vaccine research and development,which provided technical support for strengthening the prevention and control of H9N2 subtype AIV.【Method】A total of 36 H9N2 subtype AIV strains isolated in Guangxi from 2000 to 2020 were used as experimental materials.Twelve representative H9N2 subtype AIV strains from different years,different regions and different host sources were selected according to the HA genetic evolution analysis.The cross hemagglutination inhibition test(HI)and antigenicity analysis were carried out with the commercial H9N2 subtype AIV vaccine strain(SS strain)currently used.According to the results of antigenicity analysis,the representative strains with different antigenic differences were selected to prepare oil emulsion inactivated vaccine and tested for cross-immunoprotection with the commercial H9 subtype AIV vaccine strain(SS strain),respectively.【Result】Among 36 strains of H9N2 subtype AIV in Guangxi,31 strains belonged to Y280-Like(9.4.1),of which 26 strains belonged to branch I,5 strains belonged to branch II,and the other 5 strains belonged to G1-Like(h9.4.1),which were different from the evolutionary branch of the commonly used vaccine strains in China.The HA titer of 12 H9N2 subtype AIV representa-tive isolates in Guangxi after inactivation was≥7 log2,and the virus inactivation effect was good,which could be used to prepare inactivated oil emulsion vaccine,and the prepared inactivated oil emulsion vaccine had good stability and safety.The antigen correlation value(R)of each strain was calculated according to the titers of cross-HI antibody.The results showed that there was no obvious difference in antigenicity between A/chicken/Guangxi/C227/2015(H9N2 strain)(re-ferred to as C227 strain)and other strains,and the corresponding R fluctuated between 0.72 and 0.93.The results of vac-cine cross-protection test also showed that the immune protection rat

关 键 词：禽流感病毒(AIV) H9N2亚型 油乳灭活疫苗 抗原相关性 疫苗交叉保护 

分 类 号：S852.659.5[农业科学—基础兽医学]