睡眠调节相关褪黑素对类风湿关节炎滑膜细胞的作用  

Effects of Sleep-regulated Melatonin on Synovial Cells in Rheumatoid Arthritis

作　　者：常乐 孙祯 蔡振煜 翁晓源 董学峰 CHANG Le;SUN Zhen;CAI Zhenyu;WENG Xiaoyuan;DONG Xuefeng(Department of Internal Medicine,Quanzhou Medical College,Quanzhou 362000,China;Department of Orthopedics,Quanzhou First Hospital Affiliated to Fujian Medical University,Quanzhou 362000,China)

机构地区：[1]福建泉州医学高等专科学校内科学教研室,泉州362000 [2]福建医科大学附属泉州第一医院骨科,泉州362000

出　　处：《世界睡眠医学杂志》2024年第2期470-472,共3页World Journal of Sleep Medicine

基　　金：泉州医学高等专科学校校级课题青年科技(XJK1922B)。

摘　　要：目的:分析褪黑素对白细胞介素-17(IL-17)刺激下类风湿关节炎滑膜细胞活性及相关蛋白Rankl、Bcl-2和Bax表达水平的影响。方法:将类风湿关节炎滑膜细胞分为对照组、IL-17组及IL-17+褪黑素组,分别使用CCK8试剂盒以及蛋白质印迹实验检测细胞活性及相关蛋白Rankl、Bcl-2及Bax表达水平。结果:与对照组比较,IL-17组有更高的细胞活性(P<0.01);而IL-17+褪黑素组的细胞活性低于IL-17组(P<0.01)。与对照组比较,IL-17组的Rankl、Bcl-2蛋白表达水平上升,Bax表达水平下降(P<0.01);与IL-17处理组比较,IL-17+褪黑素组的Rankl、Bcl-2表达水平则下降,Bax表达水平上升(P<0.01)。结论:褪黑素能抑制IL-17对类风湿关节炎滑膜细胞的活性作用,同时褪黑素能减弱IL-17对类风湿关节炎滑膜细胞表达Rankl以及抗凋亡作用。Objective:To analyze the effect of melatonin on the activity of synovial cells and the expression of related proteins Rankl,Bcl-2 and Bax in rheumatoid arthritis stimulated by interleukin-17(IL-17).Methods:Rheumatoid arthritis synovial cells were divided into control group,IL-17 group and IL-17+melatonin group.Cell activity and expression levels of related proteins Rankl,Bcl-2 and Bax were detected by CCK8 kit and western blotting.Results:Compared with the control group,the IL-17 group had higher cell activity(P<0.01).The cell activity of IL-17+melatonin group was lower than that of IL-17 group(P<0.01).Compared with the control group,the expression levels of Rankl and Bcl-2 proteins in the IL-17 group increased,while the expression levels of Bax decreased(P<0.01).Compared with the IL-17 treatment group,the expression levels of Rankl and Bcl-2 in the IL-17+melatonin group decreased,and the expression level of Bax increased(P<0.01).Conclusion:Melatonin can inhibit the activity of IL-17 on rheumatoid arthritis synovial cells,and melatonin can reduce the expression of Rankl and anti apoptosis of IL-17 on rheumatoid arthritis synovial cells.

关 键 词：类风湿关节炎 白细胞介素-17 褪黑素 睡眠 昼夜规律 

分 类 号：R593.22[医药卫生—内科学] R338.63[医药卫生—临床医学]