基于PTEN与JAK对PI3K/Akt信号通路的影响探讨加味小蓟饮子治疗急性放射性膀胱炎的作用机制  

Exploring the Effect of Jiawei Xiaoji Yinzi on the PI3K/Akt Signaling Pathway Based on PTEN and JAK in the Treatment of Mechanism of Action of Acute Radiation Cystitis

作　　者：张伟平[1] 吴晓静 黄章铖 陈慧军 郑伟杰 翁剑飞[1] ZHANG Weiping;WU Xiaojing;HUANG Zhangcheng;CHEN Huijun;ZHENG Weijie;WENG Jianfei(The Second People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fujian Fuzhou 350003)

机构地区：[1]福建中医药大学附属第二人民医院,福建福州350003

出　　处：《深圳中西医结合杂志》2024年第6期9-14,I0002,共7页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：福建省卫生健康省级补助专项(C2023001-财政专项);福建省省直单位教育和科研补助专项(X2021006-财政专项)。

摘　　要：目的:探讨加味小蓟饮子治疗急性放射性膀胱炎的作用机制。方法:50只ICR小鼠随机分为空白组12只和造模组38只。采用X射线辐照仪造模,造模后随机取2只小鼠处死鉴定模型是否成功。将剩余造模组小鼠随机分为模型组、小蓟饮子组和加味小蓟饮子组各12只。空白组、模型组、小蓟饮子组、加味小蓟饮子组分别予0.9%氯化钠、0.9%氯化钠、小蓟饮子药液,加味小蓟饮子药液灌胃处理,每日1次,连续7 d。末次灌胃后24 h处死取材,酶联免疫吸附实验(ELISA)法检测血清超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC)含量。Western Blot法检测小鼠膀胱丝氨酸/苏氨酸激酶(AKT)、E钙粘着蛋白(E-Cadherin)、内皮素-1(ET-1)、JAK激酶(JAK)、核转录因子红系2相关因子2(Nrf2)、磷脂酰肌醇3-激酶(PI3K)、人第10号染色体缺失的磷酸酶(PTEN)、Smad蛋白2(Smad2)、Smad蛋白3(Smad3)、转化生长因子β1(TGF-β1)、尿斑蛋白3(Upk3)、血管内皮生长因子(VEGF)蛋白相对表达水平;实时荧光免疫定量-聚合酶链式反应法(RT-PCR)法检测小鼠膀胱微RNA-21(MicroRNA-21,miR-21)、PTEN、JAK、PI3K、AKT、Nrf2信使核糖核酸(mRNA)相对表达水平。结果:相较模型组,加味小蓟饮子组小鼠体内SOD、GSH-Px、T-AOC、AKT、E-Cadherin、JAK、Nrf2、PI3K、Upk3含量升高(P<0.05),MDA、ET-1、PTEN、Smad2、Smad3、TGF-β1、VEGF含量下降(P<0.05);与模型组和小蓟饮子组比较,加味小蓟饮子miR-21 mRNA表达升高(P<0.05)。结论:加味小蓟饮子组可能通过上调miR-21表达,同调PTEN、JAK蛋白以激活下游PI3K/AKT/Nrf2信号通路治疗急性放射性膀胱炎。Objective To investigate the mechanism of action of Jiawei Xiaoji Yinzi in the treatment of acute radiation cystitis.Methods Fifty ICR mice were randomly divided into 12 blank group and 38 modeling group.X-ray irradiator was used for modeling,and 2 mice were randomly selected and euthanized to identify whether the model was successful.The remaining mice in the modeling group were randomly divided into 12 mice in the model group,12 mice in the xiaojiyinzi group and 12 mice in the Jiawei Xiaoji Yinzi group.The blank group,the model group,the xiaojiyinzi group,and the Jiawei Xiaoji Yinzi group were treated by gavage with 0.9%NaCl,0.9%NaCl,xiaojiyinzi liquid,and Jiawei Xiaoji Yinzi liquid,respectively,once a day for 7 consecutive days.Samples were taken from mice at 24 h after the last gavage,and the enzyme-linked immunosorbent assay(ELISA)was used to measure the levels of serum superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-Px),total antioxidant capacity(T-AOC),Western Blot assay was used to detect mouse bladder serine/threonine kinase(AKT),E-Cadherin,endothelin-1(ET-1),JAK kinase(JAK),nuclear transcription factor red lineage 2-related factor 2(Nrf2),phosphatidylinositol 3-kinase(PI3K),human chromosome 10 deletion phosphatase(PTEN),Smad protein 2(Smad2),Smad protein 3(Smad3),transforming growth factorβ1(TGF-β1),urinary plaque protein 3(Upk3),and vascular endothelial growth factor(VEGF)protein relative expression levels;The relative expression levels of microRNA-21(miR-21),PTEN,JAK,PI3K,AKT and Nrf2 messenger RNA(mRNA)in mouse bladder were detected by real-time fluorescent immuno-quantitative polymerase chain reaction(RT-PCR).Results Compared with the model group,the contents of SOD,GSH-Px,T-AOC,AKT,Mir-21,E-cadherin,JAK,Nrf2,PI3K and Upk3 in the modified Jiawei Xiaoji Yinzi group were increased(P<0.05).The levels of MDA,ET-1,PTEN,Smad2,Smad3,Tgf-β1 and VEGF were decreased(P<0.05).Compared with the model group and the Xiaojiyinzi group,the expression of miR-21 mRNA in the Jiawei Xiaoji Y

关 键 词：放射性膀胱炎 加味小蓟饮子 PTEN JAK激酶 PI3K/AKT/Nrf2信号通路 

分 类 号：R285.5[医药卫生—中药学]