柴胡疏肝散含药血清对衣霉素诱导神经元内质网应激模型细胞凋亡的影响  

Effect of Chaihu Shugan San Containing Serum on Apoptosis in a Model of Neuronal Endoplasmic Reticulum Stress induced by Tetracycline

作　　者：李芷悦 戎志斌[2] 吕敏玲 马梦情 翟婉霞 李利民[2] 马玉翠 刘雅倩 吴正治 LI Zhiyue;RONG Zhibin;LYU Minling;MA Mengqing;ZHAI Wanxia;LI Limin;MA Yucui;LIU Yaqian;WU Zhengzhi(Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Guangdong Shenzhen 518055;The First Affiliated Hospital of Shenzhen University,Guangdong Shenzhen 518035;Shenzhen Traditional Chinese Medicine Hospital,Guangdong Shenzhen 518033)

机构地区：[1]中国科学院深圳先进技术研究院,广东深圳518055 [2]深圳大学第一附属医院,广东深圳518035 [3]深圳市中医院,广东深圳518033

出　　处：《深圳中西医结合杂志》2024年第4期5-8,I0002,I0003,共6页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金资助项目(81574038);中国博士后基金项目(2023M732373)。

摘　　要：目的:探讨柴胡疏肝散(CSS)含药血清对衣霉素诱导的内质网应激(ERS)模型神经元凋亡的影响。方法:采用衣霉素对NG108-15神经细胞进行诱导,建立ERS细胞模型并分成5组,模型组(10%空白兔血清干预)、安理申(盐酸多奈哌齐片)组(10%安理申含药血清干预)、2.5%CSS含药血清(低剂量)组、5%CSS含药血清(中剂量)组、10%CSS含药血清(高剂量)组。同时设置正常空白血清孵育且未经衣霉素诱导的正常组,干预后采用流式细胞仪和荧光染色检测各组细胞凋亡率。结果:流式细胞仪检测结果显示,各干预组细胞凋亡率均低于模型组(P <0.05);同时高剂量CSS含药血清组细胞凋亡率明显低于中低剂量组(P <0.05)。联合荧光染色也证明高剂量CSS含药血清组细胞凋亡率明显低于中低剂量组。结论:CSS能够抑制衣霉素诱导的ERS模型神经元凋亡,且其抑制作用随着药物浓度升高而增强。Objective To explore the effect of Chaihu Shugan San(CSS)containing serum on neuronal apoptosis in induced by tunicamycin of endoplasmic reticulum stress(ERS)model.Methods The NG108-15 neural cells were induced with tetracycline in the experiment,and an ERS cell model was established and divided into 5 groups:model group(10%blank rabbit serum intervention), donepezil hydrochloride table group (10% donepezil hydrochloride table drug serum intervention), 2.5% CSS drug serum (low-dose) group, 5% CSS drug serum (medium dose) group, and 10% CSS drug serum (high-dose) group. At the same time, a normal group was incubated with normal blank serum and not induced by tetracycline. After completing the observations, flow cytometry and fluorescence staining were performed to detect the apoptosis rate of cells in each group. Results The flow cytometry detection results showed that the apoptosis rate of each intervention group was significantly reduced compared to the model group (P < 0.05). At the same time, the apoptosis rate of cells in the high-dose CSS serum containing group was significantly lower than that in the medium-dose and low-dose group (P < 0.05). Combined fluorescence staining also demonstrated that the apoptosis rate of cells in the high-dose CSS serum group was significantly lower than that in the medium-close and low-dose group. Conclusion CSS has a significant protective effect on neuronal damage caused by ERS, and can inhibit neuronal apoptosis in ERS. Its inhibitory effect increases with increasing drug concentration.

关 键 词：柴胡疏肝散 含药血清 内质网应激 神经元凋亡 

分 类 号：R285.5[医药卫生—中药学]