Fine-mapping of a candidate gene for web blotch resistance in Arachis hypogaea L.  

作　　者：Xiaohui Wu Mengyuan Zhang Zheng Zheng Ziqi Sun Feiyan Qi Hua Liu Juan Wang Mengmeng Wang Ruifang Zhao Yue Wu Xiao Wang Hongfei Liu Wenzhao Dong Xinyou Zhang 

机构地区：[1]College of Agronomy,Henan Agricultural University,Zhengzhou 450046,China [2]Henan Academy of Agricultural Sciences/Henan Academy of Crop Molecular Breeding/Key Laboratory of Oil Crops in Huang-Huai-Hai Plain,Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crop Improvement,Zhengzhou 450002,China [3]The Shennong Laboratory,Zhengzhou 450002,China [4]Shangqiu Academy of Agricultural and Forestry Sciences,Shangqiu 476000,China

出　　处：《Journal of Integrative Agriculture》2024年第5期1494-1506,共13页农业科学学报（英文版）

基　　金：supported by the Key Research Project of the Shennong Laboratory,China(SN01-2022-03);the Henan Provincial Science and Technology R&D Program Joint Fund(Superiority Discipline Cultivation)Project,China(222301420100);the Major Science and Technology Projects of Henan Province,China(221100110300);the China Agriculture Research System of MOF and MARA(CARS-13);the Henan Provincial Agriculture Research System,China(S2012-5);the Outstanding Young Scientists of Henan Academy of Agricultural Sciences,China(2022YQ16);the Independent Innovation Project of the Henan Academy of Agricultural Sciences,China(2023ZC093)。

摘　　要：Peanut(Arachis hypogaea L.)is a globally important oil crop.Web blotch is one of the most important foliar diseases affecting peanut,which results in serious yield losses worldwide.Breeding web blotch-resistant peanut varieties is the most effective and economically viable method for minimizing yield losses due to web blotch.In the current study,a bulked segregant analysis with next-generation sequencing was used to analyze an F2:3 segregating population and identify candidate loci related to web blotch resistance.Based on the fine-mapping of the candidate genomic interval using kompetitive allele-specific PCR(KASP)markers,we identified a novel web blotch resistance-related locus spanning approximately 169 kb on chromosome 16.This region included four annotated genes,of which only Arahy.35VVQ3 had a non-synonymous single nucleotide polymorphism in the coding region between the two parents.Two markers(Chr.16.12872635 and Chr.16.12966357)linked to this gene were shown to be co-segregated with the resistance of peanut web blotch by 72 randomly selected recombinant inbred lines(RIL),which could be used in marker-assisted breeding of resistant peanut varieties.

关 键 词：peanut web blotch bulked segregant analysis KAsP markers resistant gene 

分 类 号：S435.652[农业科学—农业昆虫与害虫防治]