Establishment of a system for screening and identification of novel bactericide targets in the plant pathogenic bacterium Xanthomonas oryzae pv. oryzae using Tn-seq and SPR  

作　　者：Chaoyue Pang Ling Jin Haoyu Zang Damalk Saint-Claire SKoklannou Jiazhi Sun Jiawei Yang Yongxing Wang Liang Xu Chunyan Gu Yang Sun Xing Chen Yu Chen 

机构地区：[1]School of Plant Protection,Anhui Agricultural University,Hefei 230036,China [2]Institute of Plant Protection and Agro-Products Safety,Anhui Academy of Agricultural Sciences,Hefei 230031,China [3]Anhui Province Laboratory for Green Pesticide Development and Application,School of Plant Protection,Anhui Agricultural University,Hefei230036,China [4]Hefei Research Center,State Key Laboratory for Biology of Plant Diseases and Insect Pests,Hefei 230036,China [5]Key Laboratory of Integrated Crop Pest Management of Anhui Province,School of Plant Protection,Anhui Agricultural University Hefei230036,China [6]Shandong Vicome Greenland Chemical Co.,Ltd.,Jinan 250204,China

出　　处：《Journal of Integrative Agriculture》2024年第5期1580-1592,共13页农业科学学报（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China(32272587 and 32202342);the Programs for the Scientific Research Activities of Academic and Technical Leaders of Anhui Province,China(2020D251);the Development Fund for Talent Personnel of Anhui Agricultural University,China(rc342006);the University Synergy Innovation Program of Anhui Province,China(GXXT-2021-059);the Key Project of the Natural Science Foundation of Anhui Provincial Department of Education,China(2023AH040129);Anhui Province Agricultural Eco-Environmental Protection and Quality Safety Industry Technology System,China。

摘　　要：Xanthomonas spp. cause severe bacterial diseases. However, effective strategies for prevention and management of these diseases are scarce. Thus, it is necessary to improve the efficiency of control of diseases caused by Xanthomonas. In this study, Xanthomonas oryzae pv. oryzae(Xoo), which causes rice bacterial leaf blight, has been studied as a representative. A transposon insertion library of Xoo, comprising approximately 200,000 individual insertion mutants, was generated. Transposon sequencing data indicated that the mariner C9 transposase mapped at 35.7–36.4% of all potential insertion sites, revealing 491 essential genes required for the growth of Xoo in rich media. The results show that, compared to the functions of essential genes of other bacteria, the functions of some essential genes of Xoo are unknown, 25 genes might be dangerous for the Xanthomonas group, and 3 are specific to Xanthomonas. High-priority candidates for developing broad-spectrum, Xanthomonas-specific, and environment-friendly bactericides were identified in this study. In addition, this study revealed the possible targets of dioctyldiethylenetriamine using surface plasmon resonance(SPR) in combination with high performance liquid chromatography–mass spectrometry(HPLC–MS). The study also provided references for the research of some certain bactericides with unknown anti-bacterial mode of action. In conclusion, this study urged a better understanding of Xanthomonas,provided meaningful data for the management of bacterial leaf blight, and disclosed selected targets of a novel bactericide.

关 键 词：rice bacterial leaf blight XANTHOMONAS essential genes Tn-seq dioctyldiethylenetriamine 

分 类 号：TQ455[化学工程—农药化工]