Hatching and development of maize cyst nematode Heterodera zeae infecting different plant hosts  

作　　者：Jiangkuan Cui Haohao Ren BoWang Fujie Chang Xuehai Zhang Haoguang Meng Shijun Jiang Jihua Tang 

机构地区：[1]College of Plant Protection,National Key Laboratory of Wheat and Maize Crop Science,Henan Agricultural University,Zhengzhou 450002,China

出　　处：《Journal of Integrative Agriculture》2024年第5期1593-1603,共11页农业科学学报（英文版）

基　　金：This study was financially supported by the National Natural Science Foundation of China(31801717);the Major Science and Technology Projects in Henan Province,China(221100110300);the Special Fund for Young Talents in Henan Agricultural University,China(30500663);the Opening Foundation of the National Key Laboratory of Crop Science on Wheat and Maize,China(SKL2021KF06);the HAU grant for Collaborative Crop Science Research,China(CCSR2022-1)。

摘　　要：The occurrence, distribution, and rapid molecular detection technology of Heterodera zeae Koshy et al. 1971, have been reported in China. We explored the biological characteristics of H. zeae sampled in Henan Province, China to understand its interaction with plants. Cysts and second-stage juveniles(J2s) were identified under an optical and scanning electron microscope, internal transcribed spacer(ITS) phylogenetic tree, and sequence characterized amplified region(SCAR)-PCR analyses. The optimum hatching temperatures of H. zeae were 30°C and 28°C, with cumulative hatching rates of 16.5 and 16.1%, respectively, at 30 days post-hatching(dph). The hatching rate of H. zeae eggs was improved by 20-and 50-time maize soil leachate and root juice, and 10-time root exudates. The hatching rate in 10-time root exudates was the highest(25.9%). The 10-time root exudates of maize and millet produced the highest hatching rate at 30 dph(25.9 and 22.9%, respectively), followed by wheat(19.9%), barley(18.3%), and rice(17.6%). Heterodera zeae developed faster in maize than in other crops. Fourth-stage juveniles(J4s) were detected in maize roots 8 days post-inoculation(dpi) at 28°C but not in other crops. Combined with hatching tests, the Huang–Huai–Hai summer maize region and the south and central-southwest mountainous maize areas are highly suitable for H. zeae in China. This is the first systematically study of the hatching and infection characteristics on different plant hosts of corn cyst nematode H. zeae in temperate regions. This study laid a theoretical foundation for the rapid spread and high environmental adaptability of corn cyst nematode.

关 键 词：crop host resistance Heterodera zeae incubation fluid maize cyst nematode nematode development nematodehatching 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]