Low-density lipoprotein receptor-related protein 2(LRP2)is required for lipid export in the midgut of the migratory locust,Locusta migratoria  

作　　者：Yiyan Zhao Weimin Liu Xiaoming Zhao Zhitao Yu Hongfang Guo Yang Yang Hans Merzendorfer Kun Yan Zhu Jianzhen Zhang 

机构地区：[1]Research Institute of Applied Biology,Shanxi Key Laboratory of Nucleic Acid Biopesticides,Shanxi University,Taiyuan 030006,China [2]College of Life Science,Shanxi University,Taiyuan 030006,China [3]Interfaculty Institute of Cell Biology,University of Tubingen,Tubingen 72076,Germany [4]Institute of Biology,University of Siegen,Siegen 57076,Germany [5]Department of Entomology,Kansas State University,Manhattan,KS 66506,USA

出　　处：《Journal of Integrative Agriculture》2024年第5期1618-1633,共16页农业科学学报（英文版）

基　　金：supported by the National Key R&D Program of China (2022YFE0196200);the National Natural Science Foundation of China–Deutsche Forschungsgemeinschaft of Germany (31761133021);the National Natural Science Foundation of China (31970469 and 31701794);the earmarked fund for Modern Agro-industry Technology Research System, China (2023CYJSTX01-20);the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi, China (2017104);the Fund for Shanxi “1331 Project”, China

摘　　要：Low-density lipoprotein receptor-related protein 2(LRP2)is a multifunctional endocytic receptor expressed in epithelial cells.In mammals,it acts as an endocytic receptor that mediates the cellular uptake of cholesterol-containing apolipoproteins to maintain lipid homeostasis.However,little is known about the role of LRP2 in lipid homeostasis in insects.In the present study,we investigated the function of LRP2 in the migratory locust Locusta migratoria(LmLRP2).The mRNA of LmLRP2 is widely distributed in various tissues,including integument,wing pads,foregut,midgut,hindgut,Malpighian tubules and fat body,and the amounts of LmLRP2 transcripts decreased gradually in the early stages and then increased in the late stages before ecdysis during the nymphal developmental stage.Fluorescence immunohistochemistry revealed that the LmLRP2 protein is mainly located in cellular membranes of the midgut and hindgut.Using RNAi to silence LmLRP2 caused molting defects in nymphs(more than 60%),and the neutral lipid was found to accumulate in the midgut and surface of the integument,but not in the fat body,of dsLmLRP2-treated nymphs.The results of a lipidomics analysis showed that the main components of lipids(diglyceride and triglyceride)were significantly increased in the midgut,but decreased in the fat body and hemolymph.Furthermore,the content of total triglyceride was significantly increased in the midgut,but markedly decreased in the fat body and hemolymph in dsLmLRP2-injected nymphs.Our results indicate that LmLRP2 is located in the cellular membranes of midgut cells,and is required for lipid export from the midgut to the hemolymphand fat body in locusts.

关 键 词：Locusta migratoria low-density lipoprotein receptor-related protein 2 MIDGUT lipids transport RNAi 

分 类 号：S433.2[农业科学—农业昆虫与害虫防治]