水稻胚芽鞘树脂半薄大切片有效捞片和展片的方法探讨  

Effective method study of fishing and spreading based on the large resin semi-thin sections of rice coleoptile tissue

作　　者：吴佳楠 魏潜 孙莹璐 张丽娜[1] WU Jianan;WEI Qian;SUN Yinglu;ZHANG Lina(Major Platform Center,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院作物科学研究所重大平台中心,北京100081

出　　处：《电子显微学报》2024年第2期219-230,共12页Journal of Chinese Electron Microscopy Society

基　　金：中国农业科学院基本科研业务费专项(No.Y2022PT18)。

摘　　要：常规半薄切片均采用捞片环将切片从水槽中捞起置于玻片上,烤片后进行染色观察。而对于水稻胚芽鞘组织等大面积的半薄切片的制备,因为切片面积较大和水的表面张力作用,往往会造成切片褶皱和水印,从而影响细胞结构的观察。本实验将捞片环改为捞片笔,使用捞片笔将切片从槽液中捞出,置于溶度为0.001%Tween20或0.0005%Tween20的展片液上,烤干后染色,从而获得了平整无褶皱、无水印、无污染的切片,完整展现了细胞结构。同时,作者对比Tween20、Chaps和SDS等水溶液的展片效果,发现Tween20展片平整无污染、SDS展片存在褶皱和染液残留、Chaps只能保证局部展片平整。Conventionally,semi-thin sections are lifted from a water tank and placed on glass slides by a scooping ring,and then stained and observed after baking the slices.However,the traditional extracting and spreading method usually causes the fold and watermark of these sections for the preparation of large sections of the rice coleoptile tissue due to the large section area and the surface tension of water.In this work,the slices were lifted from the grooving liquid by a scooping pen that replaced the scooping ring.The slices were then placed on the scooping liquid with a concentration of 0.001% or 0.0005% Tween20,which were dyed after being dried.Based on this method,smooth slices without folds,watermarks and pollution-free were obtained with complete cell structures.By comparing the folds in Tween20,Chaps and SDS,we found that the fold in Tween20 was the most flat and non-polluting,while SDS had wrinkles and dye residue,and Chaps could only ensure the local developing flatness.

关 键 词：水稻胚芽鞘 树脂半薄切片 

分 类 号：Q-33[生物学] Q246Q942.4