BrrTCP4b interacts with BrrTTG1 to suppress the development of trichomes in Brassica rapa var. rapa  

作　　者：Cheng Li Li Zhang Hefan Li Yuanwen Duan Xuemei Wen Yongping Yang Xudong Sun 

机构地区：[1]The Germplasm Bank of Wild Species,Yunnan Key Laboratory of Crop Wild Relatives Omics,Institute of Tibetan Plateau Research at Kunming,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China [2]University of Chinese Academy of Sciences,Beijing,China [3]Tibet Plateau Institute of Biology,Lhasa 850001,Tibet,China

出　　处：《Plant Diversity》2024年第3期416-420,共5页植物多样性（英文版）

基　　金：supported by the Strategic Priority Research Program of the Chinese Academy of Sciences,Pan-Third Pole Environment Study for a Green Silk Road(Pan-TPE)(XDA2004010306);the Second Tibetan Plateau Scientific Expedition and Research(STEP)program(2019QZKK0502);Science and Technology Program of Xizang Autonomous Region(XZ202001ZY0003G).

摘　　要：The number of trichomes significantly increased in CRISPR/Cas9-edited BrrTCP4b turnip(Brassica rapa var.rapa)plants.However,the underlying molecular mechanism remains to be uncovered.In this study,we performed the Y2H screen using BrrTCP4b as the bait,which unveiled an interaction between BrrTCP4b and BrrTTG1,a pivotal WD40-repeat protein transcription factor in the MYB-bHLH-WD40(MBW)complex.This physical interaction was further validated through bimolecular luciferase complementation and co-immunoprecipitation.Furthermore,it was found that the interaction between BrrTCP4b and BrrTTG1 could inhibit the activity of MBW complex,resulting in decreased expression of BrrGL2,a positive regulator of trichomes development.In contrast,AtTCP4 is known to regulate trichomes development by interacting with AtGL3 in Arabidopsis thaliana.Overall,this study revealed that BrrTCP4b is involved in trichome development by interacting with BrrTTG1 in turnip,indicating a divergence from the mechanisms observed in model plant A.thaliana.The findings contribute to our understanding of the regulatory mechanisms governing trichome development in the non-model plants turnip.

关 键 词：TCP transcription factor MBW complex Trichome development TURNIP 

分 类 号：S631.3[农业科学—蔬菜学]