Populational change of CD4^(+)CD25^(+)Treg cells is responsible for the synergistic effect of the combination of RAMP2 with baicalin in treating recurrent spontaneous abortion mouse models  

作　　者：Cong Chen Zhuo-Lan Li Jing-Tian Guo Wen-Yao Xue Wei Guo 

机构地区：[1]Department of Gynecology,Jiangsu Province Hospital of Chinese Medicine,Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China [2]Jiangsu Key Laboratory of Druggability of Biopharmaceuticals,State Key Laboratory of Natural Medicines,School of Life Science and Technology,China Pharmaceutical University,Nanjing 211198,China

出　　处：《Traditional Medicine Research》2024年第8期59-66,共8页TMR传统医学研究

基　　金：the National Natural Science Foundation of China(81973221);National Natural Science Foundation for Young Scientists of China(81603647);the Women and Children Health Talent Project of Jiangsu Province(FRC201785);the Chinese Clinical Medicine Innovation Center of Obstetrics,Gynecology,and Reproduction in Jiangsu Province(ZX202102);the Women and Children Health Science Foundation of Jiangsu Province(F202206).

摘　　要：Background: The absence of a safe and effective therapy for recurrent spontaneous abortion due to a maternofetal failure in immunological tolerance remains an intractable clinical obstacle for surgeons. Recently, traditional Chinese medicine has become a feasible alternative for certain diseases, including recurrent spontaneous abortion. However, because of the complex composition of the traditional Chinese medicine formula, its action mechanism remains unclear. Methods: We selected two isolated active ingredients (RAMP and baicalin) from the traditional Chinese medicine formula and used an abortion-prone CBA/J × DBA/2 model to simulate human RSA and compared the changes in fetal resorption rate, Treg cell percentage, and relevant cytokines before and after combination therapy. In addition, The mechanisms were preliminarily discussed using in vitro differentiation models. Results: In CBA/J × DBA/2 abortion-prone mice, the combination therapy resulted in a lower embryo resorption rate compared to that obtained with individual delivery of either RAMP or baicalin, thereby playing an embryo-protective role through the increase in Treg cells for the maintenance of maternal-fetal immune tolerance. In in vitro primary cell differentiation experiments, the concentration of Treg cells significantly increased from 11% to 17.9% after the combination therapy compared to that of the single administration group. Conclusion: the synergistic effects of RAMP and baicalin were responsible for Treg differentiation. The present study provides a solid basis for improving the applicability of traditional Chinese herbs in the treatment of recurrent spontaneous abortion.

关 键 词：recurrent spontaneous abortion Atractylodes macrocephala Koidz. Scutellaria baicalensis Georgi CBA/J×DBA/2 regulatory T cells 

分 类 号：R714.21[医药卫生—妇产科学]