3株内生菌对棉花枯萎病菌拮抗效果研究  

Study on the Antagonistic Effect of Three Strains of Endophytic Bacteria on Cotton-wilt Fusarium

作　　者：祖丽胡玛尔·买提喀迪尔 代先兴 李猛 王天舒 闫成才 牛冬冬[2] 郝海婷 Zulihumaer·Maitikadier;DAI Xian-xing;LI Meng(Key Laboratory for Comprehensive Management of Agricultural Pests in Southern Xinjiang,Tarim University,Alar,Xinjiang 843300)

机构地区：[1]塔里木大学南疆农业有害生物综合治理重点实验室,新疆阿拉尔843300 [2]南京农业大学植物保护学院,江苏南京210095

出　　处：《安徽农业科学》2024年第9期116-120,共5页Journal of Anhui Agricultural Sciences

基　　金：南京农业大学-塔里木大学科研联合基金项目(NNLH-202002);塔里木大学校长基金(TDZKJC201801);2022校级大学生创新创业训练计划项目(2023014);2022校级研究生科研创新项目(TDGRI202224)。

摘　　要：[目的]探究3株棉花内生菌对棉花枯萎病菌的拮抗效果。[方法]以棉花枯萎病菌为试材,采用平板对峙法将3株内生菌菌悬液、上清液、菌挥发物分别对峙棉花枯萎病菌研究3株内生菌拮抗效果。[结果]3株内生菌菌悬液对棉花枯萎病菌的拮抗效果为O>T>DG3-1;3株内生菌上清液对棉花枯萎病菌的拮抗效果为T>O>DG3-1;3株内生菌菌挥发物对棉花枯萎病菌拮抗效果为DG3-1>T>O。并对抑制过的枯萎病菌菌丝形态进行显微观察,结果显示,菌丝出现空腔、断裂以及细胞壁部分溶解。重新培养抑制过的枯萎病菌,枯萎病菌可以正常生长,但生长较慢。[结论]3株内生菌对枯萎病菌生长有不同程度的影响。[Objective]To explore the antagonistic effect of three endophytic bacteria in cotton against Fusarium wilt.[Method]Using the plate confrontation method,three endophytic bacterial suspensions,supernatants,and volatiles were used to study the antagonistic effects of the three endophytic bacteria against the cotton Fusarium wilt pathogen.[Result]The antagonistic effect of the three bacterial suspensions on Fusarium oxysporum was O>T>DG3-1.The antagonistic effect of the supernatant of the three bacterial strains on F.oxysporum was T>O>DG3-1;the antagonistic effect of volatiles from three strains of bacteria on F.oxysporum was DG3-1>T>O.Microscopic observation of the morphology of the mycelium that suppressed F.oxysporum showed cavities,breakage,and partial dissolution of the cell wall.Recreate the inhibited F.oxysporum,which could grow normally but slowly.[Conclusion]Three endophytic bacteria have varying degrees of influence on the growth of Fusarium wilt.

关 键 词：芽孢杆菌 根瘤菌 内生菌 棉花枯萎病 拮抗 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治]