不同产地煨葛根的鉴定及含量测定  

Identification and Content Determination of Simmered Radix Puerariae from Different Producing Areas

作　　者：胡静[1] 位为晴 邓倩 HU Jing;WEI Wei-qing;DENG Qian(School of Chemical and Pharmaceutical Engineering,Hefei Normal University,Hefei,Anhui 230601)

机构地区：[1]合肥师范学院化学与制药工程学院,安徽合肥230601

出　　处：《安徽农业科学》2024年第9期157-160,共4页Journal of Anhui Agricultural Sciences

基　　金：安徽省药食同源天然资源开发与利用工程实验室开放基金项目(YSTY2022018);安徽省大学生创新训练计划项目(S202314098087);合肥师范学院质量工程项目(2022szsfkc01,2023jyxm07)。

摘　　要：采用显微鉴别法对四川、安徽、湖北3个产地煨葛根外观形状及内部组织结构进行初步鉴定,利用薄层色谱法(TLC)鉴定煨葛根中的主要有效成分葛根素,通过高效液相色谱法(HPLC)对它们的含量进行检测,比较分析3个产地煨葛根中有效成分葛根素的含量及其差异性。结果表明,3个产地煨葛根中均含有葛根素,四川产地样品药材葛根素含量优于安徽和湖北产地,但不同产地的煨葛根中葛根素含量均符合《中国药典》标准,同时葛根素的含量与产地有一定关系。The microscopic identification method was used to preliminarily identify the external shape and internal organizational structure of simmered Radix Puerariae from Sichuan,Anhui and Hubei Provinces.Thin-layer chromatography(TLC)was applied to identify the main active ingredient puerarin in simmered Radix Puerariae,and high-performance liquid chromatography(HPLC)was used to detect the puerarin content,the content and differences of the active ingredient puerarin in simmered Radix Puerariae from three producing areas were compared and analyzed.The results showed that puerarin was present in all three producing areas of simmered Radix Puerariae.The puerarin content in the samples from Sichuan region was higher than that from Anhui and Hubei regions.However,the puerarin content in simmered Radix Puerariae from different regions met the standards of the Chinese Pharmacopoeia,and there was a certain correlation between the puerarin content and the producing areas.

关 键 词：煨葛根 产地 显微鉴别法 薄层色谱法 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]