原发性肾小球疾病患者肠道菌群与炎症因子的相关性分析  

Correlation analysis between gut microbiota and inflammatory factors in patients with primary glomerular disease

作　　者：李朝晖[1] 朱卫国[1] 刘兵[1] LI Zhaohui;ZHU Weiguo;LIU Bing(Jinan Fourth People's Hospital,Jinan 250031,China)

机构地区：[1]济南市第四人民医院,山东济南250031

出　　处：《中国病原生物学杂志》2024年第5期550-554,共5页Journal of Pathogen Biology

摘　　要：目的探析原发性肾小球疾病患者肠道菌群水平、炎症因子水平及肠道菌群与炎症因子的相关性。方法选取2021-2023年,本院接诊的62例原发性肾小球疾病患者为本次研究对象,同时选取同期60例体检健康者为健康对照组。通过电子病历系统收集本次研究对象所有临床资料,对比不同病理类型患者的临床特点。采集参与本次研究所有研究对象的粪便样本,采用光冈法进行细菌培养,计算每克粪便中乳酸杆菌、双歧杆菌、大肠埃希菌、拟杆菌、肠球菌菌落数。采集所有研究对象的静脉血,离心处理后,采用酶联免疫吸附法检测血清中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(Interleukin-6,IL-6)、白细胞介素-17(Interleukin-17,IL-17)水平。采用Pearson相关性检验分析法分析肠道菌群与炎症因子的相关性。结果62例原发性肾小球疾病患者中,45.16%为膜性肾病,25.81%为IgA肾病,11.29%为微小病变性肾病,9.68%为系膜增生性肾小球肾炎,3.23%为局灶性节段性肾小球硬化,系膜毛细血管性肾小球肾炎、新月体性肾小球肾炎、毛细血管内增生性肾小球肾炎各占比1.61%。48.39%表现为肾病综合征,32.26%表现为肾炎综合征,14.52%表现为肾功能不全,4.84%表现为隐匿性肾炎。膜性肾病患者与IgA肾病两组患者的年龄、性别、尿酸水平差异无统计学意义(P>0.05),白蛋白水平、肌酐水平差异有统计学意义(P<0.05)。疾病组患者乳酸杆菌丰度为(5.89±0.87)LogN/g,双歧杆菌丰度为(6.45±0.98)LogN/g,大肠埃希菌丰度为(9.59±1.20)LogN/g,拟杆菌丰度为(6.89±0.95)LogN/g,肠球菌丰度为(8.71±1.29)LogN/g,健康对照组乳酸杆菌丰度为(7.43±0.99)LogN/g,双歧杆菌丰度为(9.62±1.37)LogN/g,大肠埃希菌丰度为(6.61±0.99)LogN/g,拟杆菌丰度为(8.72±0.79)LogN/g,肠球菌丰度为(7.18±0.95)LogN/g。两组患者乳酸杆菌、双歧杆菌、大肠埃希菌、拟杆菌、肠球菌丰Objective To explore the levels of gut microbiota,inflammatory factors,and the correlation between gut microbiota and inflammatory factors in patients with primary glomerular disease.Methods 62 patients with primary glomerular disease admitted to our hospital from 2021 to 2023 were selected as the study subjects,while 60 healthy individuals who underwent physical examinations during the same period were selected as the healthy control group.All clinical data of the study subjects were collected by our hospital's electronic medical record system and the clinical characteristics of patients with different pathological types were compared.The fecal samples were collected from all participants in this study,the light column method was used for bacterial culture,and the number of Lactobacillus,Bifidobacterium,Escherichia coli,Bacteroidetes,and Enterococcus colonies per gram of feces were calculated.The venous blood were collected and centrifuged from all study subjects,and the levels of the tumor necrosis factor-α(TNF-α),Interleukin-6(IL-6)and interleukin-17(IL-17)in serum were detected by enzyme-linked immunosorbent assay.The correlation between gut microbiota and inflammatory factors were analyzed by Pearson correlation analysis method.Results Among 62 patients with primary glomerular disease,45.16%had membranous nephropathy,25.81%had IgA nephropathy,11.29%had minimal degenerative nephropathy,9.68%had mesangial proliferative glomerulonephritis,3.23%had focal segmental glomerulosclerosis,and mesangial capillary glomerulonephritis,crescentic glomerulonephritis,and intracapillary proliferative glomerulonephritis each accounted for 1.61%.48.39%presents with nephrotic syndrome,32.26%with nephritis syndrome,14.52%with renal insufficiency,and 4.84%with occult nephritis.There was no statistically significant difference in age,gender,and uric acid levels between patients with membranous nephropathy and IgA nephropathy(P>0.05),while there was a statistically significant difference in albumin and creatinine levels(P<0.05).Th

关 键 词：原发性肾小球疾病 肠道菌群 炎症因子 

分 类 号：R692.6[医药卫生—泌尿科学]