基于数据挖掘探讨针灸治疗癫痫核心穴位的作用机制  

Analysis of mechanism of core points in acupuncture and moxibustion treatment for epilepsy based on data mining

作　　者：张译文 程光宇[2] 王轩[3] 程为平[3] ZHANG Yi-wen;CHENG Guang-yu;WANG Xuan;CHENG Wei-ping(The First Clinical Medical College,Heilongjiang University of Chinese Medicine,Harbin 150040,China;The Sixth Department of Acupuncture and Moxibustion,The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040;The Second Department of Acupuncture and Moxibustion,The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040)

机构地区：[1]黑龙江中医药大学第一临床医学院,哈尔滨150040 [2]黑龙江中医药大学附属第一医院针灸六科,哈尔滨150040 [3]黑龙江中医药大学附属第一医院针灸二科,哈尔滨150040

出　　处：《针刺研究》2024年第4期415-423,共9页Acupuncture Research

基　　金：黑龙江省自然科学基金项目(No.LH2021H091);黑龙江省中医药科研项目(No.ZHY19-021);双一流及优势特色学科建设项目(No.HLJSYL1006)。

摘　　要：目的:利用数据挖掘方法浅析针灸治疗癫痫的选穴规律,并探讨其核心穴方产生作用的分子机制,为针灸治疗癫痫的临床选穴和基础研究提供参考和依据。方法:以中国知网、万方数据库、中国生物医学文献数据库、维普期刊数据库及Embase、PubMed、Cochrane Library、EBSCO、Web of Science数据库中收录的相关研究文献为数据来源,对选用腧穴进行描述性分析、高频穴组分析、核心穴方挖掘与分析,并进一步对核心穴方进行潜在靶点挖掘、“核心穴方-靶点-癫痫”网络构建、蛋白质相互作用(PPI)网络建立与核心靶点提取、基因本体(GO)富集和KEGG富集分析等以预测针灸治疗癫痫的潜在机制。结果:共纳入腧穴处方122条;核心组方为百会、合谷、内关、水沟、太冲,挖掘到其潜在治疗靶点277个,与癫痫共有靶点134个,经PPI网络拓扑分析提取出核心靶点,包括信号转导和转录激活因子3、肿瘤坏死因子(TNF)、白细胞介素(IL)-6、蛋白激酶B1、c-Jun氨基末端激酶、脑源性神经营养因子、肿瘤蛋白53、血管内皮生长因子A、胱天蛋白酶3、表皮生长因子受体等;经GO和KEGG富集预测得到核心穴方治疗癫痫的主要途径,包括脂质与动脉粥样硬化、神经退行性变、磷脂酰肌醇-3-激酶-丝氨酸/苏氨酸激酶B信号通路、丝裂原活化蛋白激酶信号通路、环磷酸腺苷信号通路、TNF信号通路、IL-17信号通路、低氧诱导因子-1信号通路、凋亡等,涉及神经元死亡、氧化应激、突触可塑性、炎性反应等。结论:针灸干预癫痫的核心穴位处方可通过作用于多靶点、多通路协同发挥治疗癫痫的作用,本研究可为后续针灸治疗癫痫的临床应用及机制研究提供理论基础。Objective To explore the mechanism of core points in acupuncture and moxibustion treatment for epilepsy by using data mining technique,so as to provide a reference for clinical practice and experimental research.Methods The data comes from relevant documents collected from CNKI,Wanfang,SinoMed,VIP,PubMed,Embase,Cochrane Library,EBSCO,Web of Science databases.The selected acupoints were analyzed in descriptive statistics,high-frequency acupoints group and core acupoint prescription.Further,potential target mining,“core acupoint prescription-target-epilepsy”network construction,protein-protein interactions(PPI)network establishment and core target extraction,gene ontology(GO)and KEGG gene enrichment analysis of the core acupoint prescription were carried out to predict its anti-epileptic potential mechanism.Results A total of 122 acupoint prescriptions were included.The core acupoint prescriptions were Baihui(GV20),Hegu(LI4),Neiguan(PC6),Shuigou(GV26)and Taichong(LR3).277 potential targets were identified,among which 134 were shared with epilepsy.The core targets were extracted by PPI network topology analysis,including signal transducer and activator of transcription 3,tumor necrosis factor(TNF),interleukin(IL)-6,protein kinase B1,c-Jun N-terminal kinase,brain-derived neurotrophic factor,tumor protein 53,vascular endothelial growth factor A,Caspase-3,epidermal growth factor receptor,etc.The main anti-epileptic pathways of the core acupoints were predicted by KEGG enrichment,including lipid and atherosclerosis,neurodegeneration,phosphatidylinositol-3-kinase/protein B kinase signaling pathway,mitogenactivated protein kinase signaling pathway,cyclic adenosine monophosphate signaling pathway,TNF signaling pathway,IL-17 signaling pathway,hypoxia-inducible factor-1 signaling pathway,apoptosis,etc.,involving neuronal death,synaptic plasticity,oxidative stress,inflammation and other related biological process.Conclusion The core acupoint prescription of acupuncture and moxibustion intervention for epilepsy can act on

关 键 词：针灸 癫痫 数据挖掘 网络针灸学 机制预测 

分 类 号：R246.6[医药卫生—针灸推拿学]