全蝎软膏对糖尿病皮肤溃疡大鼠模型EGF、PDGF及其受体表达的影响  

Effects of Scorpion Ointment on EGF,PDGF and their Receptors Expression in Diabetic Skin Ulcer Rat Model

作　　者：回雪颖[1] 郭伟光[1] 王景[1] 张海丽[1] 刘颖 马铭堃 HUI Xue-ying;GUO Wei-guang;WANG Jing;ZHANG Hai-li;LIU Ying;MA Ming-kun(Department of Peripheral Vascular Diseases,The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin,Heilongjiang,150001,China)

机构地区：[1]黑龙江中医药大学附属第二医院周围血管病科,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2024年第4期630-635,共6页Progress in Modern Biomedicine

基　　金：黑龙江省卫健委项目(20210404130180);黑龙江省中医药管理局项目(ZHY2020-142);黑龙江中医药大学附属第二医院孙申田青年人才基金项目(2020KY-02)。

摘　　要：目的:探讨全蝎软膏对糖尿病皮肤溃疡大鼠模型EGF、PDGF及其受体表达的影响及创面愈合的机理。方法:60只大鼠随机分为对照组、模型组、湿润烧伤膏组和全蝎软膏组,每组15只。建立糖尿病皮肤溃疡大鼠模型,干预后7天及14天,观察并比较大鼠创面愈合率和血清中表皮生长因子(epidenllal giiilllh factor,EGF)、血小板衍生生长因子(platelet-derived growth factor,PDGF)及其受体水平。结果:全蝎软膏组、湿润烧伤膏组与模型组7 d、14 d同时间点比较创面愈合率,差异有统计学意义(P<0.05或P<0.01);全蝎软膏组、湿润烧伤膏组7 d、14 d同时间点比较,差异无统计学意义(P>0.05)。免疫组化结果显示,EGF的表达水平,全蝎软膏组、湿润烧伤膏组与模型组比较,7 d差异有统计学意义(P<0.05);14 d差异无统计学意义(P>0.05)。EGFR的表达水平,全蝎软膏组与湿润烧伤膏组、模型组、空白组7 d、14 d同时间点比较,差异无统计学意义(P>0.05)。PDGFB的表达水平,全蝎软膏组、湿润烧伤膏组与模型组7 d、14 d同时间点比较,差异有统计学意义(P<0.05)。PDGFRB的表达水平,7 d全蝎软膏组与湿润烧伤膏组、模型组、空白组比较,差异无统计学意义(P>0.05);14 d全蝎软膏组、湿润烧伤膏组与模型组比较,差异有统计学意义(P<0.05或P<0.01)。结论:全蝎软膏可影响糖尿病皮肤溃疡大鼠模型EGF、PDGF及其受体的表达,促进糖尿病创面愈合。Objective:To investigate the effect and the mechanism of wound healing of Scorpion ointment on the expression of EGF,PDGF and their receptors in diabetic skin ulcer rat model.Methods:Sixty rats were randomly divided into control group,model group,MEBO group and scorpion ointment group,with 15 rats in each group.Diabetic skin ulcer rat model was established,7 and 14 days after intervention,wound healing rate and Levels of epidermal growth factor(EGF),platelet-derived growth factor(PDGF)and their receptors in serum were observed and compared.Results:The wound healing rates of scorpion ointment group,MEBO group and model group were compared at the same time point at 7 d and 14 d,and the difference was statistically significant(P<0.05 or P<0.01);there was no significant difference between scorpion ointment group and MEBO group at 7 d and 14 d at the same time point(P>0.05).Immunohistochemical results showed that there were statistically significant differences in the expression levels of EGF between scorpion ointment group,MEBO group and model group at 7 d(P<0.05),but there was no statistically significant difference at 14 d(P>0.05).There was no significant difference in the expression level of EGFR between scorpion ointment group and MEBO group,model group and blank group at 7 d and 14 d at the same time point(P>0.05).The expression level of PDGFB in scorpion ointment group,MEBO group and model group was statistically significant at the same time point on day 7 and day 14(P<0.05).There was no significant difference in the expression level of PDGFRB between scorpion ointment group and MEBO group,model group,blank group(P>0.05),but there was statistical significance at 14 d scorpion ointment group and MEBO group compared with model group(P<0.05 or P<0.01).Conclusion:Scorpion ointment can affect the expression of EGF,PDGF and their receptors in diabetic skin ulcer rat model,and promote the healing of diabetic wound.

关 键 词：全蝎软膏 糖尿病皮肤溃疡 表皮生长因子 血小板衍生生长因子 

分 类 号：R587.2[医药卫生—内分泌] R244.9[医药卫生—内科学] R243[医药卫生—临床医学]