云南重楼的皂苷类化学成分研究  

Chemical constituents of steroidal saponins from Paris polyphylla var.yunnanensis

作　　者：陈文文 郑淑娴 胡晋铭 田韵远 曹宇 陆云阳 刘杨 汤海峰 CHEN Wen-wen;ZHENG Shu-xian;HU Jin-ming;TIAN Yun-yuan;CAO Yu;LU Yun-yang;LIU Yang;TANG Hai-feng(Department of Chinese Materia Medica and Natural Medicines,Air Force Medical University,Xi’an 710032;School of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang Shaanxi 712046)

机构地区：[1]空军军医大学药学系中药与天然药物学教研室,西安710032 [2]陕西中医药大学药学院,陕西咸阳712046

出　　处：《中南药学》2024年第4期851-856,共6页Central South Pharmacy

基　　金：国家自然科学基金面上项目(No.81973192);陕西省重点研发计划重点产业创新链(No.2021ZDLSF04-07);陕西省中医药管理局“秦药”开发重点科学研究项目(No.2021-02-ZZ-003)。

摘　　要：目的 研究云南重楼根状茎中的皂苷类化学成分。方法 利用硅胶柱色谱、硅胶C-18柱色谱、Sephadex LH-20凝胶柱色谱以及半制备高效液相色谱等技术,从云南重楼根茎70%乙醇提取物的水饱和正丁醇萃取物中,纯化得到单体成分。通过NMR、MS等波谱分析和酸水解等化学方法对其进行结构鉴定。结果 共分离得到6个皂苷类化合物,分别鉴定为parisyunnanosideⅠ(1)、paristenoside B(2)、26-O-β-D-吡喃葡萄糖基-3β,16α,26-三羟基-(23R,25R)-16,23-环胆甾烷醇-5,17(20)-二烯-22-酮-3-O-α-L-呋喃阿拉伯糖基-(1→4)-[α-L-吡喃鼠李糖基-(1→2)]-β-D-吡喃葡萄糖苷(3)、26-O-β-D-吡喃葡萄糖基-17,22-二羟基-(25R)-呋甾-5-烯-3-O-α-L-吡喃鼠李糖基-(1→2)-β-D-吡喃葡萄糖苷(4)、parisyunnanoside A(5)和26-O-β-D-吡喃葡萄糖基-3β,26-二羟基-(25R)-胆甾-5,17(20)-二烯-16,22-二酮-3-O-α-L-吡喃鼠李糖基-(1→4)-[α-L-吡喃鼠李糖基-(1→2)]-β-D-吡喃葡萄糖苷(6)。结论 化合物3、4和6为首次从云南重楼中发现。Objective To determine the steroidal saponin constituents in rhizomes of Paris polyphylla var.yunnanensis.Methods C-18 silica gel column chromatography and semi-preparative HPLC were used to isolate and purify the 70%ethanol extract from the rhizomes of Paris polyphylla var.yunnanensis.The structures of the isolated compounds were determined by extensive spectroscopic analysis and chemical methods.Results Six compounds were isolated and identified as parisyunnanosideⅠ(1),paristenoside B(2),26-O-β-D-glucopyranosyl-3β,16α,26-trihydroxy-(23R,25R)-16,23-cyclocholest-5,17(20)-dien-22-one-3-O-α-L-arabinofuranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside(3),26-O-β-D-glucopyranosyl-17,22-dihydroxy-(25R)-furost-5-en-3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside(4),parisyunnanoside A(5),and 26-O-β-D-glucopyranosyl-3β,26-dihydroxy-(25R)-cholesta-5,17(20)-dien-16,22-dione-3-O-α-L-rhamnopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside(6).Conclusion Compounds 3,4 and 6 have been isolated from the rhizomes of Paris polyphylla var.yunnanensis for the first time.

关 键 词：云南重楼 皂苷 化学成分 

分 类 号：R284.1[医药卫生—中药学]