高效液相色谱法测定α-硫辛酸绿茶片中α-硫辛酸的含量  

Determination ofα-lipoic acid inα-lipoic acid with green tea tablets by high-performance liquid chromatography

作　　者：徐硕[1] 王月 邝咏梅[1] 徐文峰[1] 金鹏飞[1] XU Shuo;WANG Yue;KUANG Yong-mei;XU Wen-feng;JIN Peng-fei(Department of Pharmacy,Beijing Hospital,National Center of Gerontology,Institute of Geriatric Medicine,Chinese Academy of Medical Sciences,Beijing Key Laboratory of Assessment of Risk and Individual Application of Clinical Drugs(Beijing Hospital),Beijing 100730)

机构地区：[1]北京医院药学部,国家老年医学中心,中国医学科学院老年医学研究院,北京市药物临床风险与个体化应用评价重点实验室(北京医院),北京100730

出　　处：《中南药学》2024年第4期1065-1069,共5页Central South Pharmacy

摘　　要：目的 建立α-硫辛酸绿茶片中α-硫辛酸的含量测定方法。方法 利用高效液相色谱(HPLC)技术,采用Alltima C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸溶液(55∶45),流速为1.0 mL·min^(-1),检测波长为215 nm,柱温为30℃。结果 α-硫辛酸在102.6~307.7μg·mL^(-1)与峰面积呈良好的线性关系,相关系数(r)为1.000;精密度、重复性和稳定性考察RSD均小于2.0%;3个浓度的加样回收率为98.44%~100.2%,RSD值均小于1.0%。结论 该方法准确、简单、快速,能够用于α-硫辛酸绿茶片中α-硫辛酸的含量测定。Objective To establish a method for the determination ofα-lipoic acid inα-lipoic acid with green tea tablets.Methods HPLC was carried out,and the experiment was conducted with an Alltima C_(18) column(250 mm×4.6 mm,5μm).The mobile phase was acetonitrile-0.1%phosphoric acid solution(55∶45)at the flow rate of 1.0 mL·min^(-1).The detection wavelength was 215 nm and the column temperature was 30℃.Resultsα-lipoic acid showed a good linearity with the peak area at 102.6~307.7μg·mL^(-1),and the correlation coefficient(r)was 1.000.The RSD of precision,repeatability and stability were less than 2.0%.The average recovery of 3 concentrations was 98.44%~100.2%,and the relative standard deviation was less than 1.0%.Conclusion The method is fast,simple and accurate,and can be used to determineα-lipoic acid inα-lipoic acid with green tea tablets.

关 键 词：Α-硫辛酸 α-硫辛酸绿茶片 高效液相色谱 含量测定 

分 类 号：R917[医药卫生—药物分析学] R927[医药卫生—药学]