A novel tube for sperm density gradient centrifugation procedure  

作　　者：Yun-Jing Xue Wei Du Cun-Zhong Deng Yuan Xu Chen-Chen Liu Yan-Nan Yang Ling Wang Yu-Zhen Lv Zheng Li Ri-Cheng Chian 

机构地区：[1]Center for Reproductive Medicine,Shanghai 1Oth People's Hospital of Tongji University,Shanghai 200072,China [2]Center for Reproductive Medicine,Women's and Children's Hospital of Jiaozuo City,Jiaozuo 454001,China [3]Department of Andrology,Center for Men's Health,Shanghai General Hospital,Shanghai 200080,China [4]Department of ART,Shanghai General Hospital,Shanghai200080,China.

出　　处：《Reproductive and Developmental Medicine》2023年第4期224-229,共6页生殖与发育医学（英文版）

基　　金：the National Key R&D Program of China(2017YFC1002003)to Z.L.and R.C.C.

摘　　要：Objective:Density gradient centrifugation(DGC),swim up(SU),or a combination of DGC and SU is used to prepare sperm for infertility treatments using assisted reproductive technologies(ARTs).This study aimed to investigate the efficiency of a new device for detecting sperm DGC.Methods:Sperm samples were collected from 119 males who required semen analysis at three differentin vitro fertilization(IVF)centers(71 samples from Center A,28 samples from Center B,and 20 samples from Center C).Semen samples were divided into two groups for DGC:(1)a normal centrifugation tube and(2)a specially designed RC-Tube.The sperm concentration,motility,and DNA fragmentation index(DFI)were assessed using DGC.Sperm concentration and motility were assessed using a MAKLER counting chamber.Sperm DFI was measured and analyzed using flow cytometry using a DFI Kit.Results:There were no significant differences in sperm concentrations recovered following DGC between the two groups(31.1%±1.7%vs.28.8%±1.3%).Sperm motility was not significantly different following the DGC procedure in both groups(77.6%±1.6%.vs.75.8%±1.6%).The percentage of sperm DFI was also not significantly different following DGC between the two groups(10.4%±1.0%vs.10.5%±1.0%).Conclusions:The newly designed RC-Tube is efficient for sperm DGC and can be used to simplify sperm preparation for infertility treatment.

关 键 词：SPERM CENTRIFUGATION MOTILITY DFI RC-Tube 

分 类 号：R714.8[医药卫生—妇产科学]