聚合物纤维载体对L929细胞的增殖及毒性研究  

Study on Proliferation and Cytotoxicity of Polymeric Fiber Carrier on L929 Cells

作　　者：李长金 李杰[1] 李晓敏 张朝旭 张正 郭子芳[1] Li Changjin;Li Jie;Li Xiaomin;Zhang Zhaoxu;Zhang Zheng;Guo Zifang(SINOPEC(Beijing)Research Institute of Chemical Industry Co.,Ltd.,Beijing,100013)

机构地区：[1]中石化(北京)化工研究院有限公司,北京100013

出　　处：《现代塑料加工应用》2024年第2期5-8,共4页Modern Plastics Processing and Applications

基　　金：中国石化科技部项目(222099)。

摘　　要：研究了进口聚合物纤维载体(1#)与国产聚合物纤维载体(2#和3#)对L929细胞增殖与毒性的影响。用CCK-8法在不同浸提液含量、不同培养时间下检测L929细胞在聚合物纤维载体中的相对增殖率。按通用标准评价材料的细胞毒性,并对载体表面和内部的细胞黏附性进行表征。结果表明:浸提液质量分数在6.25%~100.00%时,1#组、2#组、3#组均没有潜在细胞毒性,1#组、2#组、3#组的细胞毒性级别分别为“1”“0”和“1”级。体外细胞培养试验表明:在24 h的培养时间内,2#组、3#组的细胞增殖率和1#组基本接近;48~72 h的培养时间内,2#组、3#组的细胞增殖率略低于1#组。细胞黏附试验表明:2#组、3#组的细胞增殖率高于1#组,且细胞倾向于在纤维载体的内部生长。国产聚合物纤维载体的生物相容性、细胞毒性以及细胞黏附性均达到理想效果,其中3#组聚合物纤维载体的细胞增殖和细胞黏附效果最理想。The effects of imported polymeric fiber carriers(1#)and domestic polymeric fiber carriers(2#and 3#)on the proliferation and toxicity of L929 cells were studied.The relative proliferation rate of L929 cells in polymeric fiber carrier was detected by CCK-8 method at different extract concentration and culture time.The cytotoxicity of the material was evaluated according to general criteria,and the cell adhesion on the surface and inside of the carrier was characterized.The results show that there is no potentia cytotoxicity in groups 1#,2#and 3#when the mass fraction of the extract was between 6.25%and 100.00%,and the cytotoxicity of groups 1#,2#and 3#are graded as“1”“0”and“1”,respectively.In vitro cell culture test shows that the cell proliferation rate of group 2#and group 3#is similar to that of group 1#within 24 h.The cell proliferation rate of group 2#and group 3#is slightly lower than that of group 1#within 48~72 h.The cell adhesion test shows that the cell proliferation rate of group 2#and group 3#is higher than that of group 1#,and the cells tend to grow in the interior of the fiber carrier.The biocompatibility,cytotoxicity and cell adhesion of the domestic polymeric fiber carrier are all satisfactory,and the cell proliferation and cell adhesion of the 3#polymeric fiber carrier are the best.

关 键 词：纤维载体 细胞毒性 L929细胞 CCK-8试验 

分 类 号：Q813.11[生物学—生物工程] TQ342[化学工程—化纤工业]