腐植酸水溶肥对柴胡Bupleurum chinense幼苗生长及其生理特性的影响  

Effects of humic acid water-soluble fertilizer on growth and physiological characteristics of Bupleurum chinense seedlings

作　　者：彭云霞[1,2] 王国祥 蔡子平[1,2] 武伟国 白波[1,2] 米永伟 PENG Yun-xia;WANG Guo-xiang;CAI Zi-ping;WU Wei-guo;BAI Bo;MI Yong-wei(Institute of Chinese Herbal Medicines,Gansu Agricultural Sciences,Lanzhou 730070,China;Gansu Provincial Engineering Laboratory for Genetic Improvement and Quality Control of Chinese Herbal Medicine,Lanzhou 730070,China)

机构地区：[1]甘肃省农业科学院中药材研究所,甘肃兰州730070 [2]甘肃省中药材种质改良与质量控制工程实验室,甘肃兰州730070

出　　处：《中国中药杂志》2024年第7期1802-1808,共7页China Journal of Chinese Materia Medica

基　　金：甘肃省农业科学院重点研发项目(2021GAAS05);中央引导地方科技发展资金项目(22ZY1QA001);农业农村部农业科技创新能力条件建设项目(2109-00000020-01-199092);兰州市人才创新创业项目(2023-RC-24)。

摘　　要：该研究以“中柴1号”为实验材料,应用盆栽方式研究腐植酸水溶肥对柴胡苗期生长及生理特性的影响。采用单因素试验设计,柴胡苗龄为60 d时分别设腐植酸水溶肥稀释倍数1200(T1)、1500倍液(T2)、1800倍液(T3)、2100倍液(T4)对幼苗进行处理,以清水为对照(CK),处理30 d后分析各处理对柴胡幼苗形态指标、生物量积累、根系活力、抗氧化酶活性、膜脂过氧化作用以及光合特性等的影响。结果表明:与CK相比,T3处理的株高、叶片数、根粗、根长分别显著增加了36.82%、37.03%、42.78%、22.38%,根鲜重、叶鲜重、根干重、叶干重相较CK分别极显著增加了90.36%、98.68%、123.84%、104.38%。此外,腐植酸水溶肥还增强柴胡幼苗根系2,3,4-氯化三苯四氮唑(TTC)还原活力,抑制丙二醛(MDA)含量,增加超氧化物歧化酶(SOD)、过氧化物酶(POD)及过氧化氢酶(CAT)等酶活性,增加叶绿素含量,提升Pn、Gs、Tr等光合参数。综上可知,施用腐植酸水溶肥1800倍液可显著促进柴胡幼苗生长、增强根系活力、提高幼苗抗逆能力、增强光合作用,该研究结果可为柴胡苗期施肥提供理论依据。The effects of humic acid water-soluble fertilizer on the growth and physiological characteristics of Bupleurum chinense seedlings(Zhongchai No.1)were studied by using a single factor experiment design.When the seedling age was 60 days,the humic acid water-soluble fertilizer was diluted 1200 times(T1),1500 times(T2),1800 times(T3),and 2100 times(T4)for seedling treatment,respectively,and water was used as the control(CK).The effects of different treatments on growth indexes,biomass accumulation,root activity,antioxidant enzyme activity,membrane lipid peroxidation,and photosynthetic characteristics of B.chinense seedlings were analyzed after 30 days.The results showed that compared with CK,stem height,leaf number,root diameter,and root length of the B.chinense seedlings under T3 treatment were significantly increased by 36.82%,37.03%,42.78%,and 22.38%,respectively.Root fresh weight,leaf fresh weight,root dry weight,and leaf dry weight under T3 treatment were significantly increased by 90.36%,98.68%,123.84%,and 104.38%,respectively.In addition,humic acid water-soluble fertilizer also enhanced TTC reducing activity of the root of B.chinense seedlings,inhibited malonaldehyde(MDA)content,increased superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)enzyme activities,improved chlorophyll content,and enhanced Pn,Gs,Tr,and other photosynthetic parameters.In conclusion,the application of humic acid water-soluble fertilizer diluted 1800 times can significantly promote the growth of B.chinense seedlings,enhance root vitality,improve seedling stress resistance,and enhance photosynthesis.The results of this study can provide a theoretical basis for fertilization of B.chinense seedlings.

关 键 词：柴胡 腐植酸水溶肥 幼苗生长 根系活力 生理特性 光合作用 

分 类 号：S567.79[农业科学—中草药栽培]