基于天竺黄中15种蛋白质氨基酸含量分析的营养价值评价  

Evaluation of nutritional value of Bambusae Concretio Silicea based on content of 15 amino acids

作　　者：孔祥河 任广喜 徐裕彬 姜丹[1] 刘春生[1] KONG Xiang-he;REN Guang-xi;XU Yu-bin;JIANG Dan;LIU Chun-sheng(Beijing University of Chinese Medicine,Beijing 102488,China;Hebei Jujing Pharmaceutical Co.,Ltd.,Anguo 071200,China)

机构地区：[1]北京中医药大学,北京102488 [2]河北橘井药业有限公司,河北安国071200

出　　处：《中国中药杂志》2024年第7期1809-1817,共9页China Journal of Chinese Materia Medica

基　　金：中央高校横向课题项目(2020110031001469);国家自然科学基金项目(82173921)。

摘　　要：通过PITC柱前衍生化-HPLC测定天竺黄中15种蛋白质氨基酸的含量,结果发现15种总蛋白质氨基酸(TAA)质量分数为0.61~12.25 mg·g^(-1),此15种蛋白质氨基酸中平均含量排名前五的是天冬氨酸(Asp)、谷氨酸(Glu)、脯氨酸(Pro)、甘氨酸(Gly)和缬氨酸(Val)。必需蛋白质氨基酸(EAA)质量分数为0.24~4.75 mg·g^(-1),儿童必需蛋白质氨基酸(CEAA)质量分数为0.30~4.73 mg·g^(-1),非必需蛋白质氨基酸(NEAA)质量分数为0.40~7.50 mg·g^(-1),药用蛋白质氨基酸(MAA)质量分数为0.36~6.51 mg·g^(-1),呈味蛋白质氨基酸中甜味(SAA)、苦味(BAA)、鲜味(FAA)和无味(OAA)蛋白质氨基酸质量分数分别为0.22~4.70、0.19~4.03、0.13~2.26、0.06~1.26 mg·g^(-1),甜味蛋白质氨基酸含量最高,苦味蛋白质氨基酸次之。21批天竺黄中蛋白质氨基酸成分组成一致,但其含量却存在显著差异。在3个产地中,广东产地的总蛋白质氨基酸(TAA)含量、必需蛋白质氨基酸(EAA)含量、儿童必需蛋白质氨基酸(CEAA)含量、非必需蛋白质氨基酸(NEAA)含量、药用蛋白质氨基酸(MAA)含量、呈味蛋白质氨基酸含量均是最高。运用氨基酸比值(RAA)、氨基酸比值系数(RCAA)、氨基酸比值系数分(SRCAA)进行天竺黄中蛋白质氨基酸的营养价值评价,结果表明,广东产地的天竺黄营养价值更优。基于天竺黄中15种蛋白质氨基酸含量分析的营养价值评价,以期为天竺黄的质量等级评价提供数据支撑,也为其资源的开发利用奠定基础。The content of 15 total amino acids(TAAs)in Bambusae Concretio Silicea was determined by HPLC with phenylisothiocyanate(PITC)for pre-column derivatization.The results showed that the content of TAA was 0.61-12.25 mg·g^(-1),and aspartic acid(Asp),glutamic acid(Glu),proline(Pro),glycine(Gly),and valine(Val)were the top five amino acids in terms of the average content.The content of essential amino acids(EAAs),conditionally essential amino acids(CEAAs),non-essential amino acids(NEAAs),and medicinal amino acids(MAAs)was 0.24-4.75,0.30-4.73,0.40-7.50,and 0.36-6.51 mg·g^(-1),respectively.Among the delicious amino acids,sweet amino acids(SAA),bitter amino acids(BAA),fresh-taste amino acids(FAAs),and odourless amino acids(OAAs)had the content of 0.22-4.70,0.19-4.03,0.13-2.26,and 0.06-1.26 mg·g^(-1),respectively.The 21 batches of Bambusae Concretio Silicea samples presented the same composition but significant differences in the content of amino acids.Among the three producing areas,Guangdong was the area where the samples had the highest content of TAAs,EAAs,CEAAs,NEAAs,MAAs,and delicious amino acids.Furthermore,the ratio of amino acid(RAA),ratio coefficient of amino acid(RCAA),and score of ratio coefficient of amino acid(SRCAA)were calculated to evaluate the nutritional value of Bambusae Concretio Silicea.The results showed that the Bambusae Concretio Silicea samples from Guangdong had better nutritional value.The nutritional value evaluation based on the content of 15 amino acids was proposed to provide data support for the quality grading of Bambusae Concretio Silicea and lay a foundation for the development and utilization of the medicinal material resources.

关 键 词：天竺黄 异硫氰酸苯酯(PITC) 柱前衍生化 蛋白质氨基酸含量 营养价值评价 

分 类 号：R284.1[医药卫生—中药学]