MALDI coupled with laser-postionization and trapped ion mobility spectrometry contribute to the enhanced detection of lipids in cancer cell spheroids  

作　　者：Jing Chen Peisi Xie Pengfei Wu Yu He Zian Lin Zongwei Cai 

机构地区：[1]Ministry of Education Key Laboratory of Analytical Science for Food Safety and Biology,Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety,College of Chemistry,Fuzhou University,Fuzhou 350116,China [2]Department of Chemistry,State Key Laboratory of Environmental and Biological Analysis,Hong Kong Baptist University,Hong Kong,China [3]College of Forestry,Nanjing Forestry University,Nanjing 210018,China

出　　处：《Chinese Chemical Letters》2024年第4期464-468,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos.22036001, 22276034 and 22106130)。

摘　　要：Cancer cell spheroids(CCS) are a valuable three-dimensional cell model in cancer studies because they could replicate numerous characteristics of solid tumors. Increasing researches have used matrix-assisted laser desorption/ionization mass spectrometry imaging(MALDI-MSI) to investigate the spatial distribution of endogenous compounds(e.g., lipids) in CCS. However, only limited lipid species can be detected owing to a low ion yield by using MALDI. Besides, it is still challenging to fully characterize the structural diversity of lipids due to the existence of isomeric/isobaric species. Here, we carried out the initial application of MALDI coupled with laser-postionization(MALDI-2) and trapped ion mobility spectrometry(TIMS) imaging in HCT116 colon CCS to address these challenges. We demonstrated that MALDI-2 is capable of detecting more number and classes of lipids in HCT116 colon CCS with higher signal intensities than MALDI. TIMS could successfully separate numerous isobaric/isomeric species of lipids in CCS. Interestingly, we found that some isomeric/isobaric species have totally different spatial distributions in colon CCS. Further MS/MS imaging analysis was employed to determine the compositions of fatty acid chains for isomeric species by examining disparities in signal intensities and spatial distributions of product ions. This work stresses the robust ability of TIMS and MALDI-2 imaging in analyzing endogenous lipids in CCS, which could potentially become powerful tools for future cancer studies.

关 键 词：Matrix-assisted laser desorption/ionization(MALDI) Mass spectrometry imaging MALDI coupled with laser-postionization Trapped ion mobility spectrometry Cancer cell spheroids 

分 类 号：O657.3[理学—分析化学] R730[理学—化学]