Coupling WO_(3-x) dots-encapsulated metal-organic frameworks and template-free branched polymerization for dual signal-amplified electrochemiluminescence biosensing  

作　　者：Fei Yin Erli Yang Xue Ge Qian Sun Fan Mo Guoqiu Wu Yanfei Shen 

机构地区：[1]Medical School,Southeast University,Nanjing 210009,China [2]Center of Clinical Laboratory Medicine,Zhongda Hospital,Southeast University,Nanjing 210009,China [3]Jiangsu Provincial Key Laboratory of Critical Care Medicine,Southeast University,Nanjing 210009,China

出　　处：《Chinese Chemical Letters》2024年第4期469-474,共6页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos.22074015 and 22174014)。

摘　　要：Developing accurate and sensitive DNA methyltransferase(MTase) analysis methods is essential for early clinical diagnosis and development of antimicrobial drug targets. In this work, by coupling WO_(3-x) dotsencapsulated metal-organic frameworks(MOFs) as co-reactants and terminal deoxynucleotidyl transferase(Td T)-mediated template-free branched polymerization, a dual signal-amplified electrochemiluminescent(ECL) biosensor was constructed to detect DNA adenine methylation(Dam) MTase. The employment of WO_(3-x) dots-encapsulated MOFs(i.e., NH_(2)-UIO66@WO_(3-x) ) was not only beneficial for biomolecule conjugation because of the abundant amino groups but also led to a 7-fold enhanced ECL response due to the increased loading of WO_(3-x). Moreover, Td T-mediated template-free branched polymerization promoted the capture of ECL emitters on the electrode surface, achieving 20-fold enhanced signal amplification. The presented ECL biosensor demonstrated a low detection limit of 2.4 × 10^(-4)U/m L, and displayed high reliability for the detection of Dam MTase in both spiked human serum and E. coli cell samples, and for the screening of potential inhibitors. This study opens a new avenue for designing a dual signal amplificationbased ECL bioassay for Dam MTase and screening inhibitors in the fields of clinical diagnosis and drug development.

关 键 词：Dual signal-amplified electrochemiluminescence WO_(3-x)dots MOFs Template-free branched polymerization DNA methyltransferase 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.1[化学工程—制药化工] Q503[理学—分析化学]