Visible-light-activated aziridination reaction enables simultaneous resolving of C=C bond location and the sn-position isomers in lipids  

作　　者：Qiongqiong Wan Yanan Xiao Guifang Feng Xin Dong Wenjing Nie Ming Gao Qingtao Meng Suming Chen 

机构地区：[1]The Institute for Advanced Studies,Renmin Hospital of Wuhan University,Wuhan University,Wuhan 430072,China

出　　处：《Chinese Chemical Letters》2024年第4期481-485,共5页中国化学快报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China (Nos.22074111, 22004093 and 22004092);the National Key Research and Development Program of China (No.2021YFC2700700);the support of the start-up funds of Wuhan University and the National Youth Talents Plan of China。

摘　　要：There is a close relationship between the biological functions of lipids and their structures, and various isomers greatly increases the complexity of lipid structures. The C=C bond location and sn-position are two of the essential attributes that determine the structures of unsaturated lipids. However, simultaneous identification of both attributes remains challenging. Here, we develop a visible-light-activated aziridination reaction system, which enables the dual-resolving of the C=C bond location and sn-position isomerism of in lipids when combines with liquid chromatography-mass spectrometry(LC-MS). Based on the derivatization of C=C bonds with Ph I=NTs, their location in lipids could be easily identified by tandem MS. Especially, the sn-position isomers of unsaturated phosphatidylcholine(PC) can be separated and quantified by LC-MS after the derivatization. By using the proposed method, the significant changes of the sn-position isomers ratios of PC in mouse brain ischemia were revealed. This study offers a powerful tool for deep lipid structural biology.

关 键 词：AZIRIDINATION Lipid isomers C=C bond location sn-Position Mass spectrometry 

分 类 号：O657.63[理学—分析化学] O641.2[理学—化学]