Nano-sized stationary phase packings retained by single-particle frit for microchip liquid chromatography  

作　　者：Wangyan Hu Ke Li Xiangnan Dou Ning Li Xiayan Wang 

机构地区：[1]Center Excellence for Environmental Safety and Biological Effects,Beijing Key Laboratory for Green Catalysis and Separation,Department of Chemistry,Beijing University of Technology,Beijing 100124,China [2]Center for Environmental Metrology,National Institute of Metrology,Beijing 100029,China

出　　处：《Chinese Chemical Letters》2024年第4期486-489,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No.21936001);the Beijing Outstanding Young Scientist Program (No.BJJWZYJH01201910005017)。

摘　　要：Modern chromatography is increasingly focused on miniaturization and integration. Compared to conventional liquid chromatography, microfluidic chip liquid chromatography(microchip-LC) has the potential due to its zero-dead volume connection and ease of integration. Nano-sized packings have the potential to significantly enhance separation performance in microchip-LC. However, their application has been hindered by packing difficulties. This study presents a method for packing nano-sized silica particles into a microchannel as the stationary phase. The microchip-LC packed column was prepared by combining the weir and the porous silica single-particle as frit to retain the packing particles. A surface tensionbased single-particle picking technique was established to insert porous single-particle frit into glass microchannels. Additionally, we developed a slurry packing method that utilizes air pressure to inject nano-sized packing into the microchannel. Pressure-driven chromatographic separation was performed using this nano-packed column integrated into a glass microchip. The mixture of four PAHs was successfully separated within just 8 min using a 5 mm separation channel length, achieving high theoretical plates(10~6plates/m). Overall, these findings demonstrate the potential of utilizing nano-sized packings for enhancing chromatographic performance in microchip systems.

关 键 词：Nano-size packing Single-particle frit Packed column Microchip LC Chromatographic separation 

分 类 号：O652.63[理学—分析化学] TB383.1[理学—化学]