改良CTAB法提取不同作物总RNA技术研究  

Technology of Improved CTAB Method for Extracting Total RNA from Different Crops

作　　者：刘国梅 郭淑慧 孙璇 姚琳[2] 张高扬 杜春芳[1,2] LIU Guomei;GUO Shuhui;SUN Xuan;YAO Lin;ZHANG Gaoyang;DU Chunfang(Agricultural College,Shanxi Agricultural University,Jinzhong,Shanxi 030801;Cotton Research Institute,Shanxi Agricultural University,Yuncheng,Shanxi 044000)

机构地区：[1]山西农业大学农学院,山西晋中030801 [2]山西农业大学棉花研究所,山西运城044000

出　　处：《中国农学通报》2024年第11期28-35,共8页Chinese Agricultural Science Bulletin

基　　金：山西农业大学生物育种工程项目“油菜优异种质创新及广适多样性品种选育”(YZGC010);运城市农业科技公关项目“油料作物(大豆、油菜、向日葵等)新品种选育及栽培技术集成”(2021YNZD01)。

摘　　要：为提高操作效率,本研究开发出一种简洁有效的作物总RNA提取方法。研究以油菜、棉花、花生、小麦、马铃薯、玉米、大豆、西葫芦等作物的不同组织为试材,通过CTAB法、改良CTAB法及TanSzol Reagent试剂盒法提取总RNA,检测结果发现,经过改良后的CTAB法提取的总RNA质量较高。在油菜花瓣、小麦、花生、西葫芦叶片中,该方法获得了较高的总RNA得率,分别为24.5、23.7、26.4、17.35μg/mL;OD260/280和OD260/230值分别为1.90、1.73、1.72、1.80和2.10、1.50、2.01、1.70。综合比较以上3种方法,改良后的CTAB法适用于油菜、小麦、花生、西葫芦等作物的总RNA提取,为后续分子生物学检测奠定了基础。In order to improve the operation efficiency,a simple and effective method for crop total RNA extraction was developed.Total RNA was extracted from different tissues of rape,cotton,peanut,wheat,potato,corn,soybean,zucchini and other crops by CTAB method,improved CTAB method and TanSzol Reagent kit method.The detection results showed that the quality of total RNA extracted by improved CTAB method was higher.High total RNA yields were obtained in rape petals,wheat,peanut and zucchini leaves,which were 24.5,23.7,26.4 and 17.35μg/mL,respectively;and the OD260/280 and OD260/230 values were 1.90,1.73,1.72,1.80 and 2.10,1.50,2.01,1.70,respectively.By comprehensive comparison of the above three methods,the improved CTAB method is suitable for total RNA extraction of rape,wheat,peanut,zucchini and other crops,which lays a foundation for subsequent molecular biological detection.

关 键 词：改良CTAB法 总RNA RNA提取 

分 类 号：S-3[农业科学]