Ginsenoside modified lipid-coated perfluorocarbon nanodroplets: A novel approach to reduce complement protein adsorption and prolong in vivo circulation  

作　　者：Jie Zhou Binyang Gao Huan Zhang Rui Yang Jianbo Huang Xin Li Yi Zhong Yan Wang Xiaoxia Zhu Yan Luo Feng Yan 

机构地区：[1]Ultrasound Department of West China Hospital,Sichuan University,Chengdu 610041,China [2]Laboratory of Ultrasound Imaging of West China Hospital,Sichuan University,Chengdu 610041,China [3]West China Washington Mitochondria and Metabolism Research Center,West China Hospital,Sichuan University,Chengdu 610041,China [4]Research Core Facilities of West China Hospital,Sichuan University,Chengdu 610041,China

出　　处：《Acta Pharmaceutica Sinica B》2024年第4期1845-1863,共19页药学学报（英文版）

基　　金：This work was supported by the National Natural Science Foundation for Young Scholars of China(82302199);the National Science Foundation of China(82371977,82071940);the Medical Research Project of Chengdu Municipal Health Commission(2021017,2022338,China).

摘　　要：Lipid-coated perfluorocarbon nanodroplets(lp-NDs)hold great promise in bio-medicine as vehicles for drug delivery,molecular imaging and vaccine agents.However,their clinical utility is restricted by limited targeted accumulation,attributed to the innate immune system(IIS),which acts as the initial defense mechanism in humans.This study aimed to optimize lp-ND formulations to mini-mize non-specific clearance by the IIS.Ginsenosides(Gs),the principal components of Panax ginseng,possessing complement inhibition ability,structural similarity to cholesterol,and comparable fat solubi-lity to phospholipids,were used as promising candidate IIS inhibitors.Two different types of ginsenoside-based Ip-NDs(Gs Ip-NDs)were created,and their efficacy in reducing IS recognition was examined.The Gs p-NDs were observed to inhibit the adsorption of C3 in the protein corona(PC)and the generation of SC5b-9.Adding Gs to Ip-NDs reduced complement adsorption and phagocytosis,resulting in a longer blood circulation time in vivo compared to lp-NDs that did not contain Gs.These results suggest that Gs can act as anti-complement and anti-phagocytosis adjuvants,potentially reducing non-specific clear-ance by the IS and improving lifespan.

关 键 词：Lipid-coated perfluorocarbon nanodroplets Protein corona Complement C3 PHAGOCYTOSIS Ginsenosidelipid-coated perfluorocarbon nanodroplets Innate immune system Complement system Mononuclear phagocytic system 

分 类 号：R943[医药卫生—药剂学]